Co-Silencing of the Voltage-Gated Calcium Channel β Subunit and High-Voltage Activated α(1) Subunit by dsRNA Soaking Resulted in Enhanced Defects in Locomotion, Stylet Thrusting, Chemotaxis, Protein Secretion, and Reproduction in Ditylenchus destructor.
Co-Silencing of the Voltage-Gated Calcium Channel β Subunit and High-Voltage Activated α(1) Subunit by dsRNA Soaking Resulted in Enhanced Defects in Locomotion, Stylet Thrusting, Chemotaxis, Protein Secretion, and Reproduction in Ditylenchus destructor.
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dsRNA 浸泡对电压门控钙通道 β 亚基和高压激活 α1 亚基的共同沉默导致解构茎线虫的运动、管心针推力、趋化性、蛋白质分泌和繁殖缺陷增强
DOI：
10.3390/ijms23020784
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发表时间：
2022-01-11
影响因子：
5.6
通讯作者：
Ding Z
中科院分区：
文献类型：
--
作者：
An M;Chen X;Yang Z;Zhou J;Ye S;Ding Z
电压门控钙通道（VGCC）β亚基（Cavβ）蛋白是一种细胞质辅助亚基，在调节高压激活（HVA）钙通道的表面表达和门控特性中起重要作用。腐烂茎线虫是一种重要的植物寄生线虫。本研究对D.对DdCavβ进行了分子表征。原位杂交结果表明，DdCavβ在线虫各组织中均有表达。转录分析表明，DdCavβ在果蝇各发育阶段均有表达。在第三期幼鱼中表达量最高。通过dsRNA浸泡介导的RNA干扰（RNAi）验证了DdCavβ的关键作用。单独沉默DdCavβ或HVA Cavα1以及DdCavβ和HVA Cavα1基因的共沉默导致D.析构函数HVA Cavα1和Cavβ亚基的共沉默比单基因沉默表现出更强的干扰效应。本研究为植物寄生线虫VGCC的进一步研究提供了参考。
The voltage-gated calcium channel (VGCC) β subunit (Cavβ) protein is a kind of cytosolic auxiliary subunit that plays an important role in regulating the surface expression and gating characteristics of high-voltage-activated (HVA) calcium channels. Ditylenchus destructor is an important plant-parasitic nematode. In the present study, the putative Cavβ subunit gene of D. destructor, namely, DdCavβ, was subjected to molecular characterization. In situ hybridization assays showed that DdCavβ was expressed in all nematode tissues. Transcriptional analyses showed that DdCavβ was expressed during each developmental stage of D. destructor, and the highest expression level was recorded in the third-stage juveniles. The crucial role of DdCavβ was verified by dsRNA soaking-mediated RNA interference (RNAi). Silencing of DdCavβ or HVA Cavα1 alone and co-silencing of the DdCavβ and HVA Cavα1 genes resulted in defective locomotion, stylet thrusting, chemotaxis, protein secretion and reproduction in D. destructor. Co-silencing of the HVA Cavα1 and Cavβ subunits showed stronger interference effects than single-gene silencing. This study provides insights for further study of VGCCs in plant-parasitic nematodes.
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影响因子：
3.2
作者：
Han Z;Boas S;Schroeder NE
通讯作者：
Schroeder NE
影响因子：
6.7
作者：
Crisford A;Calahorro F;Ludlow E;Marvin JMC;Hibbard JK;Lilley CJ;Kearn J;Keefe F;Johnson P;Harmer R;Urwin PE;O'Connor V;Holden-Dye L
通讯作者：
Holden-Dye L
DOI：
10.1895/wormbook.1.161.1
发表时间：
2013-08-13
期刊：
WormBook : the online review of C. elegans biology
影响因子：
--
作者：
Hobert, Oliver
通讯作者：
Hobert, Oliver
影响因子：
64.8
作者：
Herndon, LA;Schmeissner, PJ;Driscoll, M
通讯作者：
Driscoll, M
影响因子：
56.9
作者：
HORVITZ, HR;CHALFIE, M;EVANS, PD
通讯作者：
EVANS, PD