虫瘿形成过程中角倍蚜唾液蛋白的鉴定与分析

植食性昆虫的唾液在昆虫与寄主植物互作关系起着至关重要的作用。五倍子蚜虫寄生在盐肤木属植物上，通过口针的取食刺激叶组织细胞增生膨大形成虫瘿（五倍子），这些虫瘿富含单宁，是重要的医药和化工原料。本项目选取五倍子蚜虫中具有进化理论意义和较高经济价值的"角倍蚜-盐肤木"作为研究体系，采用液相色谱-质谱分析、蛋白质凝胶电泳和酶活力分析技术，结合生物信息学分析方法，从生化和分子水平研究角倍蚜虫瘿形成过程中干母和干雌唾液的蛋白质组成、唾液酶种类及其活力变化规律，阐明角倍蚜的唾液的蛋白成分及其刺激盐肤木叶片组织增生形成虫瘿的生理生化过程，研究结果对于深化角倍蚜与盐肤木的互作机制、探索角倍蚜虫瘿形成机理及开辟五倍子产量提高的新途径等具有重要的理论和实践意义。

Herbivore insect saliva plays critical roles in insect-host plant interactions and is responsible for damage associated with insect feeding. Chinese gall aphids parasitize Rhus trees and stimulate the leaf tissue cells hyperplasia, and finally form galls called Chinese gallnut. These galls are rich in tannin acid and have been widely used for chemical and medicine purposes. We choose aphid-sumac as interaction system to detect the aphid salivary protein composition and enzyme activity on the process of gall formation by Liquid Chromatography-Mass Spectrometry, protein gel electrophoresis and enzymes activity analysis. The purpose is revealing salivary protein composition, enzyme activity of the horned gall aphid, Schlechtendalia chinensis. The results may benefit to enhance the research of insect-plant interaction and insect gall formation mechanism. These works may benefit to open up new ways for increasing the yield of Chinese gallnut.