纳米技术和生物技术的引入促进了免疫诊断技术的巨大进步。然而传统的酶链免疫检测花费大量的时间在重复的孵育和洗涤过程中，借助于复杂的检测仪器而且需要对操作人员进行培训。本项目在滑动微流控技术的基础上，设计出在微流控芯片的通道中有序的通入样品和试剂的液体片段进行快速ELISA反应。申请人将设计出通过芯片滑动来自动产生ELISA反应所需要液体片段的装置，并与铂纳米粒子为探针的V－Chip相结合，将开发出一种快速，定量，可视化和操作简单的一体化即时诊断工具。申请人拟将这种一体化的芯片用于非小细胞肺癌三种标志物CEA，SCCA和Cyfra 21-1的检测中。

The introduction of nanotechnology and biotechnology results in the great improvement in enzyme-linked immunoassay (ELISA). However, the traditional ELISA spends a lot of time in the repeated washing and incubation steps, requires sophisticated instruments and well－trained operators. Based on slipchip technology, a new device will be developed for ELISA detection by delivering the sample and reagent segments in the microfluidic channel in sequence. The liquid segments can be automatically produced by sliding the chip, which can be combined with platinum nanoparticles integrated volumetric bar-chart chip for development of a rapid, quantitative, visualized, simple and highly integrated point-of-care device. This integrated device will be employed for detection of the three biomarkers of non-small cell lung cancer: CEA，SCCA and Cyfra 21-1.