肿瘤外泌体携带着肿瘤患者丰富的生物分子信息，是肿瘤液体活检重要的靶标。目前，外泌体分离和分析方法依然面临众多问题，这些问题的成功解决是实现外泌体临床应用的关键。本项目将以滑动微流控技术为基础，设计出全新的外泌体的分离纯化芯片，并且与外泌体可视化定量检测芯片相结合，建立起新的外泌体的即时诊断平台。申报人将利用DNA链置换反应，在分离芯片中实现细胞培养液和血液中的外泌体的高效分离；借助于DNA杂交链反应和级联信号放大策略，在检测芯片上实现外泌体的高灵敏度可视化检测。由于不使用复杂的检测和流体控制装置，并且样品和试剂的使用量较小，可以对多种外泌体表面蛋白标志物同时检测，因此该检测平台能够用于外泌体即时检测中。申请人将以卵巢癌为模型，发展出一套成熟的外泌体的检测系统，为外泌体的即时检测提供一条新的思路。

The tumor exosome, which carries the rich biological information of the tumor patients, is an important target for the liquid biopsy of tumor. At present, the methods for exosome separation and analysis still face many problems, and the successful solution of these problems is the key to the realization of the clinical application of exosome. Based on the technology of slipchip, we will design a new chip for separation and purification of exosomes and combine it with visual quantitation chip, to establish a new point-of-care diagnostic platform for exosome. We will use DNA strand displacement reaction to efficiently separate the exosome from cell culture medium and blood in the separation chip, and achieve high sensitivity and visual detection of exosome on chip by means of hybridization chain reaction and multistaged signal amplification strategy. As the final platform will not employ complicated detection and flow control instruments, only needs a small amount of sample and reagent, and also can be used for detection of a variety of exosome surface biomarker at the same time， the platform can be used for point-of-care detection of exosomes. We will develop a set of mature exosome detection system with ovarian cancer as a model to provide a new way for the point-of-care detection of exosome.