Trf1是一种端粒结合蛋白，其在配子发生过程中的作用机制尚不清楚。我们前期构建了生殖细胞中特异性敲除Trf1的小鼠，发现敲除Trf1导致雄生殖细胞大量丢失、精子不能形成、曲细精管内的生殖细胞大多数阻滞在减数分裂的前期。此外，我们还验证了Trf1和端粒在减数分裂前期细胞中具有共定位。因此，Trf1可能在减数分裂前期花束期的形成中发挥重要作用。本课题在前期工作基础上，运用小鼠遗传学表型分析法,从细胞和分子水平深入研究Trf1在减数分裂关键事件中的功能及机理。本课题研究工作主要集中在（1）利用已经构建的生殖细胞条件性敲除Trf1的小鼠，研究Trf1在减数分裂前期端粒结合核膜过程中的作用；（2）Trf1在减数分裂前期花束期形成过程中的作用；（3）Trf1在配子发生中功能的作用机制。本课题研究结果将进一步阐明Trf1和端粒在减数分裂过程中的功能和分子机制，为人类生殖健康和生殖疾病的治疗提供理论依据。

Trf1 is a telomere binding protein,its role in gametogenesis is totally unknown. In our previous study, we have generated germ cell specific Trf1 knockout mice in male, it was found once trf1 was ablated germ cell died quickly without sperm formation, and when Trf1 was deleted most of the germ cells in Seminiferous tubules were arrested at the prophase of the meiosis. In addition, we found Trf1 colocalized with telomere duiring the prophase of meiosis. These indicated that Trf1 may have important role in the meiotic bouquet formation in the prophase of meiosis. Based on those results, we will further investigate the function and molecular mechanism of Trf1 during gametogenesis，our work will mainly focus on (1)the role of Trf1 in telomere attachment to nuclear envelope; (2) the role of Trf1 in meiotic bouquet formation in prophase of meiosis;(3) mechanism under the function of Trf1 in gametogenesis. The future results of the project will provide not only new idea about role of telomere in meiosis but may also clinical application in reproductive health and the treatment of reproductive related diseases.