强精片修复血睾屏障损伤的RTK-PI3K/Akt-mTOR信号转导机制研究

近年来我国生育形势逐年严峻，虽有“二孩”政策等措施进行调控，但仍无法扭转生育率的下降。育龄夫妇生育能力下降是其中的重要因素，不育夫妇中约50%的男性存在精液质量异常。血睾屏障（BTB）是精子发生的基本结构，其完整性和重塑（打开、关闭和稳定）功能是维持正常生精微环境的基础。近来发现RTK-PI3K/Akt-mTOR（Rictor）信号通路可调控BTB的相关连接和蛋白结构，成为研究新方向。以“肾主生殖”理论为指导，中医药在改善精子质量方面临床疗效显著，但其作用机制尚缺乏深入、系统的研究。随着现代医学对BTB分子机制的研究进展，着力于中医药改善男性生育力的作用机制研究成为目前亟需解决的问题。本研究采用临床显效方剂强精片干预模型小鼠，通过RTK-PI3K/Akt-mTOR（Rictor）信号通路和BTB结构蛋白的“线-面”研究，以及BTB结构“开-闭”的动态观察，从不同角度探讨中医药的调控作用。

In recent years, China's fertility situation has been severe year by year. Although there are measures such as the "two-child" policy, it is still unable to reverse the decline in fertility. The decline in fertility in couples of childbearing age is an important factor, and about 50% of males in infertile couples have abnormal semen quality. The blood testis barrier (BTB) is the basic structure of spermatogenesis, and its integrity and remodeling (opening, closing, and stabilizing) functions are the basis for maintaining a normal spermatozoa. Recently, it has been found that the RTK-PI3K/Akt-mTOR (Rictor) signaling pathway can regulate the related junction and protein structure of BTB, and has become a new research direction. Guided by the theory of “kidney controlling reproductive”, Chinese medicine has a significant clinical effect in improving sperm quality, but its mechanism of action is still lacking in in-depth and systematic research. With the advancement of modern medicine on the molecular mechanism of BTB, research on the mechanism of action of Chinese medicine to improve male fertility has become an urgent problem to be solved. In this study, the clinically effective prescriptions of Qiangjing tablet is used to intervene in the model mice, the RTK-PI3K/Akt-mTOR (Rictor) signaling pathway and BTB structural proteins as the "line-surface" study and dynamic observation of "open-close" in BTB structure are carried out in order to explore the regulatory role of traditional Chinese medicine from different angles.