低强度脉冲超声LIPUS联合3D生物打印制作心肌生物补片治疗缺血性心脏病

缺血性心脏病的治疗已成为全球性挑战，现行临床手段乃至血管新生的基因治疗均不能有效解决心肌梗死时心肌细胞大量丧失的难题。近年来，3D生物打印和心肌组织工程的研究进展使基于心肌细胞移植的心肌修复和重建展现出令人期待的应用前景。本研究针对传统细胞移植时细胞留存率低，传统组织工程技术制造的组织补片携带移植细胞数量少、移植细胞存活率低的局限性，以心肌细胞作为种子细胞，海藻酸盐水凝胶复合物作为支架材料，采用先进的3D生物打印技术研制具备生物活性的心肌补片，植入到心肌梗死区进行治疗；同时，利用低强度脉冲超声LIPUS的生物学效应优化补片中3D细胞支架结构，改善移植细胞生存的微环境，籍此提高移植心肌细胞的存活率以及补片与受体心肌的兼容性，实现心肌修复重建。本研究立足于心血管临床长期存在的难题，采用医学和生物工程领域的最前沿技术，旨在克服传统技术方法的局限性，是在医工交叉领域和转化医学领域进行的有益探索。

The treatment of ischemic heart disease has become a global challenge. The current clinical methods and even the gene therapy of angiogenesis cannot effectively solve the problem of large number of myocardial cells loss during myocardial infarction. In recent years, advances in 3D bioprinting and myocardial tissue engineering have shown promising application prospects for myocardial repair and reconstruction based on myocardial cell transplantation. In order to improve the limitation of low survival rate and insufficient quantity of cell number that traditional tissue engineering cardiac patch has,this study use myocardial cells as seed cells, alginate gel as the scaffold material, combining with advanced 3D bioprinting technology to make cardiac patch for the treatment of myocardial infarction.At the same time, the biological effects of low-intensity pulsed ultrasound (LIPUS) were utilized to optimize the 3D cell scaffold structure of the patch and improve the microenvironment of the transplanted cells, so as to improve the survival rate of the transplanted cardiomyocytes and the compatibility between the patch and the recipient myocardium to achieve myocardial repair and reconstruction. Based on the long-standing problems in cardiovascular clinical practice, this study adopts the most cutting-edge technologies in the field of medicine and bioengineering, aiming to overcome the limitations of traditional technology and methods, and is a beneficial exploration in the field of medical and engineering intersection and translational medicine.