油菜BnSYP122所介导的病程相关蛋白分泌机制研究

植物病程相关蛋白的分泌机制目前还不是很清楚。前期研究中，我们发现BnPR1和BnPDF1.2可在油菜BnSYP122的过表达植株中被核盘菌侵染诱导高表达，进一步分析发现BnPR1和BnPDF1.2定位于BnSYP122包被的囊泡里。本申请项目中我们拟在此基础上，通过分析BnSYP122和其互作蛋白的功能、修饰方式、修饰位点及其对病程相关蛋白分泌的影响，明确BnSYP122介导的病程相关蛋白分泌机制，为进一步明确植物的抗病机制、改造该分泌途径提高油菜对菌核病的抗性及制定有效的病害防治策略奠定理论基础。

The secretory mechanism of plant pathogenesis-related proteins is still unclear. In previous studies, we found that pathogenesis-related protein 1 (PR1) and plant defensin (PDF) 1.2 could be highly induced in BnSYP122 over-expressed plants of Brassica napus by Sclerotinia sclerotiorum infection. Further analysis revealed that BnPR1 and BnPDF1.2 localized in vesicles enclosed by BnSYP122. On this basis, we intend to clarify the secretory mechanism of pathogenesis-related protein mediated by BnSYP122 through analyzing the function, the protein modification types and modification sites of BnSYP122 and its interacted proteins induced by S. sclerotiorum infection. Their effects on secreting pathogenesis-related proteins will be also carried out. The results can lay a theoretical foundation for further understanding the mechanism of plant resistance, and providing a new strategy to improve rapeseed resistance to S. sclerotiorum by modifying the secretory pathway.