ANGUSTIFOLIA negatively regulates resistance to Sclerotinia sclerotiorum via modulation of PTI and JA signalling pathways in Arabidopsis thaliana.
ANGUSTIFOLIA negatively regulates resistance to Sclerotinia sclerotiorum via modulation of PTI and JA signalling pathways in Arabidopsis thaliana.
复制标题
DOI：
10.1111/mpp.13222
复制
发表时间：
2022-08
影响因子：
4.9
通讯作者：
Wang, Airong
中科院分区：
文献类型：
--
作者：
Gao, Xiuqin;Dang, Xie;Yan, Fengting;Li, Yuhua;Xu, Jing;Tian, Shifu;Li, Yaling;Huang, Kun;Lin, Wenwei;Lin, Deshu;Wang, Zonghua;Wang, Airong
关键词：
核盘菌（Sclerotinia sclerotiorum）是一种广泛侵染植物的毁灭性病原菌。植物防御真菌入侵的机制仍然没有得到很好的表征。在这里，我们报告，ANGUSTIFOLIA（AN），一个CtBP家族成员，在防御S。菌核病发作拟南芥突变体对S. sclerotiorum在感染的早期阶段比野生型植物。因此，在用PAMP/微生物相关分子模式处理后，突变体表现出病原体相关分子模式（PAMP）触发的免疫（PTI）应答的更强激活，包括促分裂原活化蛋白激酶激活、活性氧物质积累、胼胝质沉积和PTI应答基因的表达。此外，过表达AN的拟南芥品系对S.并显示有缺陷的PTI反应。我们的发光测定法、双分子荧光互补、共免疫沉淀和体外下拉试验表明，AN与茉莉酸（JA）生物合成所必需的酶丙二烯氧化物环化酶（AOC）相互作用，负调节JA生物合成以响应S.菌核病这项工作揭示了AN是AOC介导的JA信号通路和PTI激活的负调节剂。狭叶草通过与丙二烯氧化物环化酶相互作用激活广泛的PTI反应，如ROS爆发、MAPK激活和胼胝质沉积，负调节植物对活体营养型和坏死营养型病原体的免疫。
Sclerotinia sclerotiorum is a devastating pathogen that infects a broad range of host plants. The mechanism underlying plant defence against fungal invasion is still not well characterized. Here, we report that ANGUSTIFOLIA (AN), a CtBP family member, plays a role in the defence against S. sclerotiorum attack. Arabidopsis an mutants exhibited stronger resistance to S. sclerotiorum at the early stage of infection than wild‐type plants. Accordingly, an mutants exhibited stronger activation of pathogen‐associated molecular pattern (PAMP)‐triggered immunity (PTI) responses, including mitogen‐activated protein kinase activation, reactive oxygen species accumulation, callose deposition, and the expression of PTI‐responsive genes, upon treatment with PAMPs/microbe‐associated molecular patterns. Moreover, Arabidopsis lines overexpressing AN were more susceptible to S. sclerotiorum and showed defective PTI responses. Our luminometry, bimolecular fluorescence complementation, coimmunoprecipitation, and in vitro pull‐down assays indicate that AN interacts with allene oxide cyclases (AOC), essential enzymes involved in jasmonic acid (JA) biosynthesis, negatively regulating JA biosynthesis in response to S. sclerotiorum infection. This work reveals AN is a negative regulator of the AOC‐mediated JA signalling pathway and PTI activation. ANGUSTIFOLIA negatively regulates plant immunity against biotrophic and necrotrophic pathogens by activating broad PTI responses, such as ROS burst, MAPK activation, and callose deposition, by interacting with allene oxide cyclases.
登录
查看更多内容
影响因子：
5.3
作者：
Gachomo EW;Jimenez-Lopez JC;Smith SR;Cooksey AB;Oghoghomeh OM;Johnson N;Baba-Moussa L;Kotchoni SO
通讯作者：
Kotchoni SO
植物病原真菌核盘菌的完整基因组序列揭示了对广泛宿主范围病原体的基因组结构的见解。
影响因子：
3.3
作者：
Derbyshire M;Denton-Giles M;Hegedus D;Seifbarghy S;Rollins J;van Kan J;Seidl MF;Faino L;Mbengue M;Navaud O;Raffaele S;Hammond-Kosack K;Heard S;Oliver R
通讯作者：
Oliver R
影响因子：
18
作者：
Betti F;Ladera-Carmona MJ;Weits DA;Ferri G;Iacopino S;Novi G;Svezia B;Kunkowska AB;Santaniello A;Piaggesi A;Loreti E;Perata P
通讯作者：
Perata P
影响因子：
3.5
作者：
Guo, Xiaomei;Stotz, Henrik U.
通讯作者：
Stotz, Henrik U.
影响因子：
6.2
作者：
Chen, Xiaoting;Liu, Jun;Lu, Guodong
通讯作者：
Lu, Guodong