肿瘤相关巨噬细胞靶向多功能氧化石墨烯纳米分子探针的构建及应用

肿瘤相关巨噬细胞（TAM）在肿瘤的侵袭、转移，以及药物耐受方面起着关键的作用。临床数据表明TAM的数量与患者的局部肿瘤复发、远端转移和差的预后呈明显相关性。TAM在肿瘤的关键作用提示以其为靶点将可能是一种有效的肿瘤治疗策略。另外，如果能够无创监测TAM的动态变化将为更好地评估肿瘤进展和评价疗效提供重要的技术手段。在本研究中，我们将设计构建一种TAM靶向的氧化石墨烯纳米分子探针，它具备核医学SPECT显像，近红外光学显像和肿瘤靶向光动力学治疗等多种功能。本研究将可能为在体无创监测TAM提供一种新型的技术手段。另外，本研究制备的新型纳米分子探针也将可能通过逆转肿瘤耐受而实现对肿瘤的精准治疗。

Tumor-associated macrophage (TAM) plays essential roles in tumor invasion and metastasis, and contribute to drug resistance. Clinical evidence suggests that TAM levels are correlated with local tumor relapse, distant metastasis, and poor prognosis in patients. The important roles of TAMs in cancers suggest that it is important to develop novel therapies that target these cells. In addition, noninvasive imaging of macrophage infiltrates would be of great help in better understanding cancer progression and in the evaluation of cancer treatment efficacy. In this project, we propose to develop and evaluate in vitro and in vivo a TAM-targeted nano-graphen oxide probe with multiple functions for SPECT, near-infrared fluorescence imaging, and tumor-specific photodynamic therapy. This study would provide a novel approach for the noninvasive imaging of TAM in vivo, and for monitoring tumor responses to therapies. The probe developed in this study would also be promising for the effective and image-guided treatment of tumors by reversing resistance to conventional therapeutics.