以一株具锰氧化和生物锰氧化物矿化活性的假单胞菌T34为对象，在测定该菌株在Mn2+诱导前后转录组活性的变化后，根据其锰氧化代谢网络和锰诱导下的物质与能量代谢流向，利用II型CRISPA-Cas9基因编辑技术沉默其基因组中与锰氧化非关联的50种基因，定向筛选锰氧化活性增强与锰氧化物矿物锰量增加的突变株，并利用其生物矿化活性和生物吸附掺杂作用，将Co2+/Ni2+/Al3+外源金属以不同配比掺杂于氧化物聚集体，和以其作为前体物在Ar气下于800度碳化，最终制备一种以MnO/C纳米晶体为基相和多相彼此掺杂并共同镶嵌于碳基质的、高势能中空多孔复合物材料。在进行扫描电镜、孔径分布与大小、比表面积、EDS、热重、拉曼光谱、XRD和XPS对聚集体进行多重表征后，测定其用于锂离子电池负极时的电池循环性能和倍率性能，使在0.1 A g-1电流密度下100个循环后的可逆放电容量大于700 mAh g-1。

Although increasingly more attention is being paid to the performance optimization of electrochemical materials based on the structural specificity of biomaterials, very limited reports exist on the fabrication of new biomaterials that are produced by biologically enzymatic reaction to improve material capabilities. The combinative biotemplate and bioconversion strategy that fabricates novel materials with a unique morphology and complicated structure has been recognized as a sustainable and environmentally friendly approach to material manufacturing. Previously, we have developed an electrochemical material using biomineralized manganese oxide aggregates from a Pseudomonas sp. T34 strain with Mn2+-oxidizing activity and the ability to form manganese oxide aggregates under Mn2+ induction, however, although the electrochemical performance was comparable to similar materials that were fabricated through chemical methods, the relatively low Mn content of the aggregates showed the necessity of improving Mn2+-oxidizing activity of T34 strain. In this proposal, we focus on the promotion of whole-cell Mn2+-oxidizing activity of T34 through genetic modification of this train. The whole-cell transcriptomics of T34 under Mn2+-inducing and Mn2+-lacking culture conditions will initially determined to allow probing the manganese oxidation network, substrate and energy metabolisms and metabolic influx, which will be referred to determine the target genes those are not relatively associated to manganese oxidation in its genome. A newly hot-spot genome-editing technology, namely Type II CRISPA_Cas9 system, will be employed to inactive total 50 different non-Mn oxidation-associated genes in T34’s genome that are selected above, and the activity-enhanced mutant strains with regard to both Mn2+-oxidation and aggregate formation with increased Mn content are screened through comparative assays. Consequently, one mutant strain with highest Mn2+-oxidizing activity and outstanding aggregate-formation capacity will be used as the parent strain as a precursor to synthesize a biocomposite that will be doped with Co, Ni and Al at different formula under mild shake-flask trial conditions, based on the biomineralization process of biogenic Mn oxides and the characteristics of metal ion subsiders through the distinctive biosorption/biooxidation activities of this train. The aggregates will be fabricated the hollow porous carbonaceous MnO-based cation-doped multiphasic biocomposites after high-temperature annealing of 800C and in the presence of Ar. Assays of HRTEM, pore size and porosity, BET specific surface area, thermogravimetric analysis, Raman spectrum, XRD (including XRD Rietveld analysis), EDS and XPS will performed to verify the phase composition and fine structures of the prepared materials. Furthermore, the cycling performance and rate performance of the fabricated materials used as the anode materials for lithium-ion batteries will compared by using different electrochemical analyses. The main aim of this proposal is at developing an anode material with the reversible discharge capacity after 100 cycles remains higher than 700 mAh g 1, at a current density of 0.1 Ag 1, which is a very good composite cycling stability and reversible specific capacity over the conventionally prepared similar materials by chemical methods. The proposed biotemplate strategy could be expanded to applications such as biocatalysis and sensor fields.