本项目基于偶氮苯基团的荧光淬灭作用和对偶氮还原酶的敏感性，拟利用“Click”化学和RAFT聚合方法，将二种连接了偶氮苯基团的近红外荧光素（ADP-azo）或聚集诱导荧光分子（TPE-azo）分别连接到结构精致的水溶性聚合物或两亲性聚合物的链端或链的中间，高效地设计合成一系列基于偶氮苯的酶响应型聚合物荧光探针。然后，考察这类聚合物溶液或聚合物组装形成的胶束对结肠内偶氮还原酶的响应性能以及作为药物载体的靶向输送性能。这项研究可以拓展偶氮苯类聚合物在生物传感、载药和细胞标记等领域中的应用，并填补这些领域的空白。

In this project, based on a fluorescence quenching of azobenzene group and its unique sensitivity to azoreductase, either near infrared fluorescein or aggregation-induced emission molecule of both bearing azobenzene groups (ADP-azo or TPE-azo) will be respectively incorporated into the end or middle of precisely-controlled water-soluble or amphiphilic polymer chains via the strategies of “Click” chemistry and reversible addition-fragmentation chain teansfer (RAFT) polymerization. As a result, a series of azobenzene-based functional polymers, which can be used as enzyme-responsive fluorescent probes, will be designed and synthesized efficiently. Then, the responsive properties to azoreductase enzyme in living colon and targeted delivery performance as a drug carrier of polymeric solution or polymeric micelles formed by self-assembly will be investigated. The study on this project will extend the applications of azobenzene-based polymers in biosensing, drug delivery and cell labeling, and fill a gap in these fields.