硼对人和动物骨代谢的作用备受关注。缺硼会影响人和动物骨发育甚至引发骨畸形，适量补硼对骨发育和骨量维持有积极作用。近年来由于骨代谢疾病的日益增长，故研究硼对骨代谢的作用机制显得日趋重要。研究表明，硼可促进机体雌激素分泌而具有类雌激素作用，而雌激素可促进TRPV6表达进而影响成骨细胞、破骨细胞对Ca2+的吸收，同时骨代谢平衡需要跨细胞Ca2+转运，提示硼可能通过类雌激素作用影响TRPV6钙通路吸收Ca2+而调节骨代谢。本研究在前期研究基础上，综合分析国内外研究结果，以TRPV6钙通路为研究核心，综合运用基因沉默、RT-PCR、IFA、免疫印迹等技术，通过建立大鼠摘除卵巢模型和沉默TRPV6基因的成骨细胞和破骨细胞模型，研究经硼处理后Ca2+吸收、TRPV6基因转录、蛋白分布与表达变化，揭示硼通过TRPV6钙通路影响骨代谢中Ca2+的吸收的作用机制，为硼在防制骨代谢疾病方面提供可靠的理论依据。

The effects of boron on bone metabolism are gradually being taken seriously. Boron deprivation in human and animals can impaire bone development even abnormal, while supplementation can improve bone development and maintain bone mass. In recent years, with increasing of the bone metabolism diseases, study of the boron effects on bone metabolism and reveal its mechanism of action has become increasingly important. Studies demonstrated that boron could affect the content of estrogen in animal body, and estrogen promoted the TRPV6 mRNA expression which could regulated Ca2+ absorption, while bone metabolism balance require Ca2+ transportation across the cells. In this study, based on previous studies and considered the domestic and foreign research, we take the TRPV6 calcium channel as study center, use gene silence, RT-PCR, IFA, immunoblotting techniques, and study the Ca2+ absorption, TRPV6 changes in gene transcription and protein expression in rat osteoblasts and osteoclasts which were suffered TRPV6 gene silence and the rats which were suffered ovariectomized, and then both cells and rats were treated by boron, all of above results will reveal the mechanism of the boron via TRPV6 calcium channel affecting Ca2+ absorption, and provide a reliable theoretical basis of boron in the prevention of bone metabolism diseases.