基于趋化因子/BMP-2骨再生材料的构建及其协同促进成骨机制

基于"特殊材料和生长因子"构建引导组织再生材料是提升现有骨修复材料活性的理想途径。为此，本项目紧扣骨组织再生过程中"靶细胞富集、分化和组织形成"等环节，以表面吸附壳聚糖微球的介孔/大孔硅基支架材料为基质，设计构建并活性组装细胞趋化因子（CCF）和BMP-2共同修饰的新型引导骨组织再生材料。通过CCF和BMP-2在壳聚糖微球和介孔中时空分布的不同实现其在体内的时序释放，同时依靠大孔支架来确保细胞长入和营养物质的输送，实现骨组织快速修复。研究将借助体外细胞迁移、量子点标记和生物学评价等，从细胞和分子水平研究生长因子的不同配伍及时序释放等对干细胞募集和分化过程的影响规律及机制；进一步结合体内异位和原位骨组织再生的研究结果，集成出CCF与BMP-2、CCF/BMP-2与支架材料协同引导骨再生的理论模型，为高质量骨修复材料的研制与临床应用提供理论依据。并研制出高活性骨组织修复材料，解决临床难题。

Fabrication of guided bone tissue regeneration materials (GBTR) based on the special functional materials and growth factors is an ideal strategy to enhance the osteoactivity of the available bone repair materials. Therefore, focusing on the key links "target cell homing, osteodifferentation and tissue formation" in the bone regeneration, with the chitosan microsphere-coated mesoporous/macroporous silicon-based scaffold as matrix, we will design and develop a new cell chemotactic factor (CCF)/BMP-2-incorporated GBTR scaffold. By controlling the spatiotemporal distribution, time-sequence release will be realized according to the need of the bone regeneration process. Together with the excellent cellular infiltration and nutrient and waste transport provided by the macroporous scaffold, high-quality bone regeneration will be achieved. With the help of in vitro cell migration and quantum dot labelling technology, the effects of the different combination and time-sequence release of CCF and BMP-2 on the cell migration , recruitment and osteodifferentation will be thoroughly investigated. Further combinated with the results of the ectopic and in-situ GBTR, the theory about the synergistic effects between CCF and BMP-2, CCF/BMP-2 and scaffold will be summarized and revealed. These study will provide theoretical basis for research on novel high quality bone repair material as well as its clinical application. Based on these studies, a new CCF/BMP-2-based GBTR will be developed to solve the clinic problem.