脂肪源干细胞结合双重生物活性纳米纤维构建功能性组织工程膀胱壁的实验研究

先天畸形或后天疾患如恶性肿瘤等常造成膀胱壁的大部分、甚至整体的丧失。组织工程的膀胱替代是最有前景的治疗策略。脂肪源干细胞（ADSC）来源丰富、提取简单，是较新颖 、颇具潜力的成人干细胞之一。研究证实，ADSC不但可分化为同胚层来源（中胚层）的的各种组织，如脂 肪、骨、软骨、平滑肌等，也具有跨系分化为不同来源胚层组织的潜力（如来源于内胚层的尿路上皮）；PLCL由乳酸（LL）和已内酯（CL）共聚而成，是柔韧性较强的可降解生物材料。本项目在原有的工作基础 之上，诱导ADSC向平滑肌和尿路上皮双向分化，并结合负载生长因子（血管内皮生长因子和神经生长因子）具有双重生物活性的PLCL纳米纤维，构建组织工程膀胱壁补片，修补大鼠膀胱缺损模型，探讨ADSC在这种双重生物活性的PLCl纳米细胞支架的支撑下，粘附、增殖、向平滑肌和尿路上皮双向分化的能力及生物活性细胞支架对新生膀胱壁的血管和神经再生促进作用。

Both congenital or acquired disorders such abnormalities, cancers ,injures and so on can lead to progressive loss of baldder wall.Tissue engineering is a promising therapeutic strategy for replacement of urinary bladder..Adipose derived stem cell(ADSC) is abundant-source ,obtained-easily and new alternative of stem cell ,and possess multilineage potential to differentiate in vitro into varieties of mesoderm cells such as adipogenic, chondrogenic, osteogenic and smooth muscle cells. It was also proved recently that ADSC can be transdifferentiated into no-mesoderm cell and tissue such as urothlium derived from .ectoderm. Poly(L-lactide)/Poly(ε-caprolactone) (PLCL) was prepared with blend of L-lactide(LL) and ε-caprolactone(CL) and ,is pliable and degradable biomaterail..This project, on the basis of our previous study, is proposed to induce ADSC to differentiate into two directions :smooth muscel cells and urothlium cells , and then incorporated with PLCL nanofiber scaffold loaded wity bioactive factors（vascular endothelial growth factor ，VEGF and Nerve Growth Factor，NGF） ,to construct a tissue engineering patch to repair bladder wall defect of a rat model.This project aims to explore the possbility of ADSC, supported by this dual-bioactivity PLCL patch ,attaching ,proliferating ,differentiating and the promotion of the angiogenesis and innervation of the new bladder wall by this bioactive scaffold.