Association of 17-β Estradiol with Adipose-Derived Stem Cells: New Strategy to Produce Functional Myogenic Differentiated Cells with a Nano-Scaffold for Tissue Engineering.
Association of 17-β Estradiol with Adipose-Derived Stem Cells: New Strategy to Produce Functional Myogenic Differentiated Cells with a Nano-Scaffold for Tissue Engineering.
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17-β雌二醇与脂肪干细胞的关联：利用组织工程纳米支架生产功能性肌原分化细胞的新策略
DOI：
10.1371/journal.pone.0164918
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Zeng X
中科院分区：
文献类型：
--
作者：
Feng C;Hu J;Liu C;Liu S;Liao G;Song L;Zeng X
绝经后女性压力性尿失禁发生率的增加与17-β雌二醇(17-雌二醇)水平的降低有关。在体外，E2也被证明能增强脂肪干细胞(ASCs)的多分化能力。然而，关于E2在组织工程治疗SUI中的潜在价值的研究很少。在本研究中，我们成功地构建了肌源性分化的血管干细胞(MD-ASCs)，并将其种植在聚丙交酯/聚己内酯电纺纳米支架上，并在体系中掺入雌二醇，目的是促进ASCs的增殖和肌源性分化。ASCs取自大鼠腹股沟皮下脂肪。观察补充雌二醇组和不补充雌激素组间充质干细胞的增殖和成肌分化，以及纳米支架材料的生物相容性。我们发现，在体外，E_2掺入促进了ASCs的增殖，最适浓度为10−9 M。E_2还使MD-ASCs的表型向以平滑肌诱导的浓缩型转化。不同分化阶段的MD-ASCs的早期(α-平滑肌肌动蛋白)、中期(钙蛋白)和晚期(肌球蛋白重链)收缩标志物的表达均被E2增强。此外，该纳米支架与MD-ASCs具有良好的生物相容性，并且E2显著促进了细胞的增殖。综上所述，这些结果表明，雌激素可以促进ASCs的增殖和肌源性分化，并可用于构建生物相容性的细胞/纳米支架。这些具有理想分化细胞的支架在组织工程中显示出良好的应用前景。
The increased incidence of stress urinary incontinence (SUI) in postmenopausal women has been proposed to be associated with a reduction in the level of 17-β estradiol (E2). E2 has also been shown to enhance the multi-differentiation ability of adipose-derived stem cells (ASCs) in vitro. However, studies on the potential value of E2 for tissue engineering in SUI treatment are rare. In the present study, we successfully fabricated myogenically differentiated ASCs (MD-ASCs), which were seeded onto a Poly(l-lactide)/Poly(e-caprolactone) electrospinning nano-scaffold, and incorporated E2 into the system, with the aim of improving the proliferation and myogenic differentiation of ASCs. ASCs were collected from the inguinal subcutaneous fat of rats. The proliferation and myogenic differentiation of ASCs, as well as the nano-scaffold biocompatibility of MD-ASCs, with or without E2 supplementation, were investigated. We demonstrated that E2 incorporation enhanced the proliferation of ASCs in vitro, and the most optimal concentration was 10−9 M. E2 also led to modulation of the MD-ASCs phenotype toward a concentrated type with smooth muscle-inductive medium. The expression of early (alpha-smooth muscle actin), mid (calponin), and late-stage (myosin heavy chain) contractile markers in MD-ASCs was enhanced by E2 during the different differentiation stages. Furthermore, the nano-scaffold was biocompatible with MD-ASCs, and cell proliferation was significantly enhanced by E2. Taken together, these results demonstrate that E2 can enhance the proliferation and myogenic differentiation of ASCs and can be used to construct a biocompatible cell/nano-scaffold. These scaffolds with desirable differentiation cells show promising applications for tissue engineering.
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影响因子：
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