角膜是位于眼球最前端的一层透明组织，较易发生外伤。角膜基质层主要由平行排列的胶原纤维构成，它们整齐的排列形态决定了角膜的高透明性。当基质层发生损伤，胶原纤维被破坏，如果重建的胶原纤维排列紊乱，愈合后的组织可能形成显著瘢痕，造成永久性视力障碍。角膜组织的破坏必然会导致局部应力场的改变，并会伴随着伤口的愈合而持续变化。以往的研究表明力学环境对软组织的重建具有显著影响，但针对角膜愈合中伤口附近的应力特征对胶原纤维排列形态的影响机制尚未明确。在本项目中，申请人拟建立角膜损伤的动物模型，对愈合过程中胶原纤维形态的变化进行持续观察；同时建立对应的有限元模型，模拟伤口附近的应力分布。通过组织观察和仿真结果的相关性分析，研究角膜愈合过程中伤口附近的应力分布对胶原纤维重建形态的影响，为探明角膜瘢痕的产生机制提供理论依据。

The cornea is the transparent tissue at the front part of the eye, which is often injured physically. The transparency of normal cornea is mainly contributed by the collagen fibrils that are arranged in parallel in the corneal stroma. When the cornea is subject to injury, the fibrils are damaged in the wound. If the neocollagen fibrils regenerated non-parallelly, a scar would form after corneal healing, leading to permanent visual obstruction. The acute breach of fibril arrangement also affects the local stress distribution in the corneal tissue, which further changes chronically with healing. Previous studies have indicated that the mechanical environment has obvious influence on collagen fibril remodeling in soft tissue healing. However, the effect of the stress distribution characteristics on the arrangement morphology of collagen fibrils in corneal healing is unknown. In this study, we will develop animal models with corneal injury to observe the arrangement morphology of collagen fibrils in the wound acutely and chronically. In addition, we will create finite element model to estimate the stress distribution near the wound. Correlation analysis will be conducted to understand the effect of local stress distribution on the remodeling of collagen fibrils, aiming to investigate the mechanism of scar formation in corneal healing.