转录因子NFI家族在发育、分化、增殖、凋亡、迁移等多种过程中发挥重要作用。NFIB属于NFI家族，其表达异常与包括乳腺癌在内的多种肿瘤发生发展密切相关。我们前期工作表明，NFIB可调控细胞S期进程并与MCM解旋酶复合体、单链结合蛋白RPA1、组蛋白伴侣FACT复合体等复制相关蛋白及组蛋白本身相互作用。据此我们提出“NFIB与组蛋白及FACT相互作用介导复制区染色质结构开放，并与RPA1及MCM协同调控细胞S期进程”的假说，将以细胞生物学、分子生物学、高通量测序等多种手段验证，并进而探讨NFIB失调与癌细胞基因组不稳定及肿瘤发生发展的关系。

The Nuclear Factor I (NFI) family of transcription factors play an important role in a wide spectrum of biological processes such as development, differentiation, proliferation, apoptosis, and invasion. NFIB, a member of the NFI family, is dysregulated in many types of cancers including breast cancer. Our preliminary data showed that NFIB regulates S phase progression in tumor cells and interacts with several important replication-related proteins such as the MCM complex, PRA1 and the FACT complex. In addition, NFIB directly interacts with histone itself. We propose that NFIB mediates the opening of chromatin structure in the replication region by interacting with histone itself and the histone chaperone FACT, which together regulate DNA replication in concert with RPA1 and MCM helicases. We will use multiple cell biology/molecular biology/high throughput sequencing methods to test our hypothesis, and further investigate the relationship between dysregulation of NFIB and cancer cell genome instability and tumor progression.