本体感觉系统可将力学信号转化为电信号并传递到中枢神经系统形成痛觉和触觉,但本体感觉神经元感受和传递力学信号的机制尚不清楚。由于感觉神经末梢微小的物理尺度，传统的实验方法很难对其进行力学加载。本项目将建立力学钳（mechano-clamp）与膜片钳同步测定技术对感觉神经末梢和胞体进行力学加载和电信号同步记录的方法，结合力敏感离子通道基因敲除小鼠来分析感觉神经元胞体加载和亚细胞层次（感觉神经末梢）加载后细胞的不同响应。阐明力敏感TRPA1和T型钙通道在感觉神经元力信号感受和转导中的作用，揭示不同直径神经元对力敏感性的不同以及力学刺激后产生不同形式电流动力学和动作电位图式的机制。这些研究将为了解细胞力学信号转导以及疼痛等疾病的发病机制提供重要的参考资料。

In the mammalian somatosensory system, mechanosensory neurons mediate the sense of touch and pain. The molechular and cellular mechanism for mechanoreception during sensations of touch and pain remains unclear. Simultaneous mechano-clamp and patch clamp technique has been used to dissect the mechanotransduction-based response.We will elucidate the relationship between cell size and the mechanosesentivity of sensory neurons. By employing T-type calcium channel and TRPA1 knocout mice to determine the role of T-type calcium channel and TRPA1 in mechanotransduction in the sensery neuron. This approach will undoubtedly accelerate our understanding of mechanotransduction mechanism in mammalian sometosensation, facilitating future pathology studies of pain.