miRNAs和mRNA基因互作网络调控小桐子抗冷性提高的分子机制

研究开发能源植物小桐子是云南省和国家的能源战略需求，低温是限制小桐子产量和分布的重要环境因素。已知miRNAs在植物对逆境胁迫的响应与适应中起重要作用，但miRNAs如何与mRNA互作形成网络来调控植物的抗冷性仍不清楚。基于前期工作基础，本项目拟研究：①低温锻炼及随后的低温胁迫过程中小桐子转录组、miRNA组及其降解组的高通量测序、验证与比较分析；②小桐子miRNAs靶基因的预测与验证；③低温锻炼提高小桐子抗冷性过程中miRNAs与mRNA基因互作网络的解析；④对小桐子抗冷性提高有重要作用的miRNAs的克隆与功能解析。研究结果将发掘一批新的小桐子miRNAs及其潜在的靶基因，获得对小桐子抗冷性提高有重要作用的miRNAs，并最终解析低温锻炼提高小桐子抗冷性过程中miRNAs与mRNA基因互作网络，以及这种miRNAs与mRNA的互作如何来调控小桐子抗冷性提高的分子机制。

Research and development of the energy plant Jatropha curcas L. is a strategical demand for Yunnan province and our country. Low temperature is a key limiting factor for development and production of J. curcas. It is well-known that microRNAs play impotant roles in response and adaptation of plants to environmental stresses, but it is little known how these interactions between microRNAs and mRNA to integrate a network to regulate chilling resistance in plants. Based on our previous work, using two population of J. curcas with different chilling resistance as experimental material, the present proposal is going to study: 1. High-throughput sequencing, verification and comparision of transcriptome, miRomics and degradome during chill-hardening and following chilling stress in J. curcas seedlings; 2. prediction and verification of microRNA-targeted genes; 3. analysis of miRNA-mRNA interaction network during chill-hardening enhanced chilling resistance in J. curcas seedlings; and 4. cloning and functional identification of miroRNAs impotant for chilling resistance. The predicted results will explore some novel microRNAs and their target genes, as well as those micorRNAs impotant for chilling resistance, and elucidate how the miRNA-mRNA interaction gene network to regulate enhancement of chilling resistance in J. curcas and their molecular machanisms.