甜菜T510品系是具有较强耐盐特性的优质种质资源，研究发现转录因子BvBHLH93基因在甜菜耐盐品系T510中特异的响应盐胁迫诱导表达。本项目将对BvBHLH93基因的功能及其参与调控T510品系耐盐性的机制进行研究。主要研究内容：1、发展BvBHLH93启动子-基因-GUS甜菜T510品系转基因材料并进行GUS染色，分析BvBHLH93基因在对照及盐胁迫条件下各组织部位的表达模式。2、通过检测BvBHLH93基因过量表达及低表达T510品系转基因植株的表型及生理指标，确定BvBHLH93基因参与调控甜菜T510品系耐盐特性及相关生理代谢途径。3、利用染色质免疫共沉淀-测序技术筛选转录因子BvBHLH93直接调控的下游基因，并对二者调控关系及其与甜菜耐盐性的关联进行研究。本项目将促进BHLH转录因子家族调控植物耐盐性及甜菜耐盐品系耐盐机理的研究。

Sugarbeet T510 strain is a kind of excellent germplasm with strong salt tolerance. The expression of transcription factor BvBHLH93 in salt tolerant strain T510 was specially induced under salt stress conditions. Our study focused on the function of BvBHLH93 and its regulation mechanism on salt tolerance of sugar beet. Firstly, Pro(BvBHLH93):CDS (BvBHLH93):GUS vector was constructed and transformed T510 strain. Furthermore, GUS staining was used to analyze the expression patterns of BvBHLH93 gene in transgenic plants under control and salt stress conditions. Secondly, we will develop transgenic plants with BvBHLH93 gene overexpression or knock down in T510. Meanwhile, phenotypic and physiological indexes of transgenic plants will be analyzed and the physiological mechanism of BvBHLH93 involved in the regulation of salt tolerance can be elucidated. Finally, downstream genes directly regulated by BvBHLH93 can be screened by the method of chromatin immunoprecipitation sequencing. The regulation relationship between BvBHLH93 and downstream genes will be studied. This project will promote the studying of BHLH transcription factor family on regulating salt tolerance of plants and the mechanism of salt tolerance in T510 strain.