致病疫霉引发的晚疫病是马铃薯毁灭性病害，该病害的防控难点在于病菌变异快导致马铃薯抗病性易丧失，因此发掘与利用广谱抗病基因有助于破解该难题。马铃薯抗病基因Rpi-vnt1通过其编码的免疫受体识别致病疫霉保守无毒蛋白Avrvnt1从而表现出良好的广谱和持久抗性，然而具体的识别机制目前尚不清楚。项目组前期工作发现Avrvnt1特异性地依赖于马铃薯甘油盐酸激酶GLYK激活Rpi-vnt1。本项目拟进一步阐明Avrvnt1、GLYK和Rpi-vnt1相互作用的结构基础，解析Avrvnt1靶向GLYK激活Rpi-vnt1广谱抗性的分子和生化机制。研究结果有望深入理解马铃薯广谱抗病基因Rpi-vnt1的作用原理，为合理改良农作物对疫霉菌的持久抗病性提供理论指导。

Late blight caused by Phytophthora infestans is a destructive potato disease. The natural resistance gene provides potato with resistance to this disease, however, the resistance could be easily broken down due to dramatic changes in Phytophthora field population. Therefore, deploying broad spectrum resistance gene will be beneficial to disease management. Rpi-vnt1 from wild potato recognizes a core P. infestans effector Avrvnt1, providing a good source of broad spectrum and durable field resistance. However, the working mechanism how Rpi-vnt1 recognizes Avrvnt1 remains unknown. We previously found that the activation of Rpi-vnt1 in the presence of Avrvnt1 requires a potato glycerate kinase (GLYK). In this proposal, we will further investigate the interactions among Avrvnt1, GLYK and Rpi-vnt1 from a structural basis. The biochemical and cell biological approaches will be applied to dissect how Avrvnt1 activates Rpi-vnt1 in a GLYK-dependent manner. The results are expected to provide practical guide to the applications of Rpi-vnt1 to confer broad spectrum resistance.