神经系统对信息的编码、整合、传递等操作的基础主要是动作电位及其传递。尽管用膜片钳电生理方法研究神经系统电信号有着极高的时间分辨率和准确性，可以准确地记录动作电位，但因其对测量样本破坏太大，且伴随着低下的测量通量和空间分辨率而难以满足神经生物学家们的需求。在此我们提出一种基于结合光遗传学工具、可遗传编码的膜电位探针以及基于算法提升电位成像采样率方法的技术，其可重构出亚毫秒及亚微米级别时空分辨率级别的动作电位爆发与传播图像。我们将研究神经元中AnkG等关键蛋白表达差异对动作电位在轴突起始节处产生和在树突上回传电位的传播速度的影响。另外，我们也将通过光介导的局部神经递质释放借助我们的成像技术和算法在单细胞的空间分辨率下研究不同的神经递质对突触后神经元树突上回传动作电位传播特征的影响。

The basis of nervous system on encoding, integrating, and transforming information is action potentials (AP) and its propagation. Now we introduce a novel technique combing optogenetic tools, genetically-encoded voltage indicators (GEVIs) and an algorithm-based voltage imaging upsampling methods for mapping the APs initiation and propagation patterns in sub-millisecond and sub-micrometer temporal-spatial resolution.In the current project, we will investigate the role of AnkG at the axon initial segement (AIS) of AP generation and back propagation (bpAP) on the dendrites. Furthermore, we will examine the influence of specific neurotransmitter to the bpAP pattens on dendrites in single-cell resolution via photo-induced site-specific neurotransmitter release.