江淮籼稻生产急需精准、快速、高效的育种技术。基因编辑特别是碱基编辑技术在水稻定向改良育种中有重要作用。但在江淮籼稻中，遗传转化困难，碱基编辑效率低下。在前期研究的基础上，本研究拟以江淮籼稻代表性品种为对象，深入挖掘参与籼稻分化能力控制的基因，并鉴定其作用机制，构建增强籼稻遗传转化和基因编辑能力的分子模块；探索DNA甲基化修饰状态参与籼稻基因编辑效率调节的内在机制，构建增强编辑效率的分子模块；针对性地改进籼稻碱基编辑工具，提高工具自身效率；探索筛选辅助基因编辑策略和单倍体辅助基因编辑策略，积极探索不受基因型限制的碱基编辑方法。在此基础上，综合各有效模块，构建江淮地区籼稻的高效碱基编辑体系。本研究将丰富对遗传转化中关键分子机制的认识，拓宽作物碱基编辑效率提升的研究策略，为水稻及其他作物定向改良提供技术支撑，在江淮和其他地区水稻育种快速改良中，具有重要的理论意义和广阔的应用前景。

Precise, rapid and efficient breeding technologies will greatly facilate the Indica rice production in Jianghuai region. Gene editing, especially base editing technology, plays an important role in rice breeding. However, genetic transformation is difficult and base editing efficiency is limited in Jianghuai Indica rice. Based on the previous research, we plan to screen and identified the genes involved in the differentiation control of Indica rice, then constructing molecular modules to enhance the genetic transformation and gene editing ability of Indica rice. We believe that the DNA methylation status is also involved in the regulation of gene editing efficiency in Indica rice. We plan to analyze the underlying menchansim and constructing molecular modules for enhancing editing efficiency. Furthermore, the toolkit itself will be improved by modifying the expressing effeciency in Indica rice, and several auxiliary strategies, indluding the genotype-not-restricted haploid system will be tested. Together, the effective modules were stacking to construct a highly efficient base editing system for Indica rice in the Jianghuai region. This study will improve the understanding of the key molecular mechanisms in Indica rice genetic transformation, broaden the research strategy of crop base editing efficiency, and provide technical support for the targeted improvement of rice and other crops. The results will acclerate the breeding improvement of Indica rice in Jianghuai and other areas.