在橡胶树(Hevea brasiliensis)中，胶乳再生能力是影响橡胶树产量的关键因素。胶乳糖酵解为胶乳再生提供了物质及能量基础，特别是为橡胶烃合成提供了碳骨架，因此与胶乳再生密切相关。磷酸果糖激酶是糖酵解代谢中的限速及关键酶，糖酵解的代谢速度取决于此酶的活性，加速糖酵解速率将增强胶乳再生能力及提高橡胶烃产量。本项目拟从转录和翻译水平研究磷酸果糖激酶基因家族的表达特性，纯化胶乳磷酸果糖激酶并了解其酶学特性，通过分子鉴定和生化特性研究，确定胶乳磷酸果糖激酶的关键成员，明确胶乳磷酸果糖激酶的调控特点及其与胶乳再生的关系。项目结果有助于阐明橡胶树胶乳糖酵解代谢的调控机理，为全面揭示橡胶树胶乳糖酵解与胶乳再生以及橡胶产量的密切相关奠定理论基础，为橡胶树产量定向分子改良提供重要的靶基因。

In Hevea brasiliensis, the regeneration of latex is the key factor in rubber production.The glycolysis of latex provide the material and energy, and is closely realted with latex regeneration. One of the key regulatory reactions in the glycolytic pathway is the conversion of fructose-6-phosphate (F-6-P) to fructose-1,6-bisphosphate (F-1,6-P2) by phosphofructokinase. In this study, the phosphofructokinase were isolated and detected in latex basing on transcription and translation level, including gene clone, regulation of gene epxression, function of gene and characteristics of enzyme. The results will reveal how and which the key phosphofructokinase genes regulate the glycolysis of latex, and further regulate the rubber synthesis and the regeneration of latex. It is hoped to obtain one or more phosphofructokinase genes that relate to rubber yield, provide important genes for molecular assistant breeding and transgene in Hevea.