烟粉虱与棉花互作的分子机理研究

烟粉虱是典型的刺吸性口器害虫，能够大量取食棉花韧皮部汁液，同时作为媒介传播双生病毒，是目前危害棉花生产的主要害虫之一。转基因bt棉花无法抵御烟粉虱的侵害。植物凝集素基因被认为是控制烟粉虱有效候选基因，但在棉花中抗虫效果不明显。因此发掘新的抗烟粉虱分子机制及其相关基因具有重要的理论和实践价值。本项目将从不同棉花材料对烟粉虱耐受性差异的分子机制及互作出发，选用对烟粉虱耐受性有明显差异的棉花材料为研究对象，系统地研究了不同品种棉花对烟粉虱胁迫后转录组的变化，同时检测烟粉虱取食前后转录组变化，从分子水平揭示棉花感虫/抗虫的分子机理，同时挖掘烟粉虱自身消化，适应棉花次生代谢产物毒害的相关机制。通过生物信息学分析，挖掘重要抗虫信号路径相关基因及烟粉虱自身必需发育，解毒相关基因。超表达植物抗性基因或者RNAi烟粉虱生存必需的基因，获得转基因抗虫棉花材料，为今后的棉花转基因抗虫品种选育提供新的策略。

Whitefly (Bemisia tabaci) B and Q types cause extensive crop damage throughout the world by feeding directly cotton phloem sap and also by vectoring many viruses, which is the major insect on cotton production nowadays. Transgenic BT cotton did not show any toxicity to whitefly and then people are seeking help from transgenic plants expressing lectin genes. However, this strategy does not work well in cotton. So, it is highly necessary to find out the new molecular mechanism and insect-resistant genes involving in cotton /whitefly interaction. In this proposal, we will evaluate the transcriptome change between whitefly-sensitive and whitefly-tolerant cotton germplasm after attack from the herbivore. The analysis of signal pathway for basic defense response of cotton plants and the tolerance mechanism of whitefly for the toxic metabolisms producing in cotton plants will be used extensively. Based on the bioinformatics analysis of the transcriptome from both cotton plants and whiteflies, several whitefly-resistant relative genes in cotton genome and toxic metabolisms tolerance /digestion genes in whitefly genome will be indentified. These genes will be used as candidate insect resistant genes for transgenic cotton plants, which will bring new hope and strategy for protect cotton from attack by whitefly as well as other phloem-feeding pests.