Transcriptome analysis reveals a comprehensive insect resistance response mechanism in cotton to infestation by the phloem feeding insect Bemisia tabaci (whitefly).
Transcriptome analysis reveals a comprehensive insect resistance response mechanism in cotton to infestation by the phloem feeding insect Bemisia tabaci (whitefly).
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转录组分析揭示了棉花对韧皮部昆虫烟粉虱（粉虱）侵染的综合抗虫反应机制
DOI：
10.1111/pbi.12554
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发表时间：
2016-10
影响因子：
13.8
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Li J;Zhu L;Hull JJ;Liang S;Daniell H;Jin S;Zhang X
烟粉虱（Bemisia tabaci）对全球棉花生产造成巨大危害。然而，关于植物如何感知和保护自己免受这种破坏性害虫的信息非常有限。本研究采用RNA‐Seq技术，比较了对粉虱具有较强抗性（HR）和敏感性（ZS）的两个棉花品种在感染后0、12、24和48 h的转录组差异。Illumina测序技术获得了大约10亿个成对末端reads。基因本体论和KEGG通路分析表明，棉花对粉虱侵害的转录应答涉及编码蛋白激酶、转录因子、代谢物合成和植物激素信号的基因。此外，基于RNA - Seq数据集构建的加权基因共表达网络表明，WRKY40和铜转运蛋白可能是调节棉花防御粉虱侵害的枢纽基因。通过病毒诱导的基因沉默（VIGS）沉默GhMPK3导致MPK‐WRKY‐JA和ET通路的抑制，并导致对粉虱的敏感性增强，这表明在RNA‐Seq分析中鉴定的候选抗虫基因是可信的，具有重要的实用性。综上所述，本研究为棉花防御白蝇侵害提供了全面的见解，并确定了几个控制韧皮部摄食害虫的候选基因。
Summary The whitefly (Bemisia tabaci) causes tremendous damage to cotton production worldwide. However, very limited information is available about how plants perceive and defend themselves from this destructive pest. In this study, the transcriptomic differences between two cotton cultivars that exhibit either strong resistance (HR) or sensitivity (ZS) to whitefly were compared at different time points (0, 12, 24 and 48 h after infection) using RNA‐Seq. Approximately one billion paired‐end reads were obtained by Illumina sequencing technology. Gene ontology and KEGG pathway analysis indicated that the cotton transcriptional response to whitefly infestation involves genes encoding protein kinases, transcription factors, metabolite synthesis, and phytohormone signalling. Furthermore, a weighted gene co‐expression network constructed from RNA‐Seq datasets showed that WRKY40 and copper transport protein are hub genes that may regulate cotton defenses to whitefly infestation. Silencing GhMPK3 by virus‐induced gene silencing (VIGS) resulted in suppression of the MPK‐WRKY‐JA and ET pathways and lead to enhanced whitefly susceptibility, suggesting that the candidate insect resistant genes identified in this RNA‐Seq analysis are credible and offer significant utility. Taken together, this study provides comprehensive insights into the cotton defense system to whitefly infestation and has identified several candidate genes for control of phloem‐feeding pests.
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影响因子：
14.9
作者：
Hu Z;Chang YC;Wang Y;Huang CL;Liu Y;Tian F;Granger B;Delisi C
通讯作者：
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影响因子：
7.4
作者：
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影响因子：
6.2
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影响因子：
3.7
作者：
Hu, Jian;De Barro, Paul;Liu, Shu-Sheng
通讯作者：
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