超高分辨率光电联合成像技术能够在纳米尺度整合生物大分子的精确定位和细胞超微结构信息，为研究分子机器在细胞内的原位结构和动态变化提供了有效手段，是生物成像领域的发展趋势。然而，由于超分辨光电双功能标记探针的缺乏，实现同尺度（如：纳米级别）光镜电镜的信息耦合以及图像的精确配准仍面临很大挑战，严重制约了超分辨光电联合成像的快速发展和广泛应用。为了解决以上问题，本项目拟发展具有单线氧光敏效应的双功能荧光蛋白，满足超分辨荧光成像需求的同时实现电镜的特异性标记，提高光学成像的空间分辨率，改进配准的精度和效率，推动超分辨光电联合成像技术的发展。开发的探针拟应用于超大复合物的拓扑结构研究、细胞器的互作机制解析的等重要科学问题的探索。

Correlative super-resolution fluorescence and electron microscopy integrates bio-macromolecular precise localization and cellular ultrastructure information at nanoscale, and provides an effective means for studying the in-situ structure and dynamic of molecular machinery, thus being the development trend in the field of biological imaging. However, due to the lack of dual-functional labelling probes, it is still a challenge to couple the information form light and electron microscopy at nanoscale and realize accurate image registration, which seriously restricts its rapid development and widespread application. To solve these problems, this project intended to develop a dual-functional fluorescent protein with singlet oxygen photosensitizing effect, which is suitable for super-resolution fluorescence microscopy and capable of specific labeling in electron microscopy, to provide details with increased spatial resolution and to improve registration efficiency and accuracy, thus promoting the development of correlative super-resolution fluorescence and electron microscopy. The engineered probe will be used in exploration of important scientific problems such as topological structure of large complex and organelle-organelle interaction mechanism.