活细胞超分辨荧光显微成像是目前超分辨成像领域的研究热点和发展趋势，但其广泛应用，尤其是对细胞内早期/短程事件的超分辨追踪仍面临一系列挑战，其中最关键的技术瓶颈为荧光蛋白探针的性质不够优化。针对以上问题，本课题拟发展折叠好、成熟快、亮度高、转化高效的新型荧光蛋白，并将其应用于细胞周期调控、神经极性发育、翻译早期的动态规律等重要科学问题的探索，以期获得这些短暂生物进程可视化的一手材料，揭示更多生命活动的本质，同时为服务人类医疗诊断提供理论依据。

Live cell super-resolution fluorescence microscopy is the research focus and trends of super-resolution imaging, however their broad application, especially the tracking of intracellular early / short-term event at ultrahigh resolution, still faces a series of challenges, and the technical bottleneck is the properties of the fluorescent protein probes are less than optimal. Thus, the purpose of the subject is to develop fast folded, photo-conversion efficient and super bright photo-convertible fluorescent protein, for exploring the mechanism of several significant biological issues include cell cycle regulation, neuronal polarity development, dynamic laws during early translation, and revealing the nature of life, while serving human medical diagnosis.