金属化学形态纳米识别- FI-HPLC/GC-ICP-MS联用分析及代谢组学研究

金属或类金属化学形态在环境中迁移转化可能引发严重的公共卫生问题，但卫生分析中金属元素化学形态的暴露识别和检测尚缺少有效方法，金属形态对健康的作用机制还不清楚。本研究针对六种指标金属的化学形态特性，匹配性合成新型纳米材料和印迹聚合物，探讨典型金属化学形态的高效选择性识别、印迹原理、吸附规律和溶出方法；构建纳米材料与金属化学形态相互作用机制模型；基于纳料材料自组装（NMs）-FI-HPLC/GC-ICP-MS/AFS/GFAAS联用技术，建立金属形态O2O化学识别和检测流程；基于代谢组学方法和NMs-uHPLC-ESI-MS-MS联用分析，研究环境暴露不同形态金属所致生物效应、代谢机制和毒作用本质。构建金属化学形态代谢指纹图谱，探明金属形态体内分布及作用规律。解析金属形态的代谢通路，发现其环境暴露代谢标志物及生物识别监测新方法。为金属毒物分析、食品安全、环境/职业卫生控制提供科学依据。

The migration and transformation of metal or metals chemical species in the environment could cause serious public health problem. But there was still lack of effective method for chemical exposure recognition and detection of metal species in health analysis, and the toxicity mechanism of metal chemical species to health was unclear. A serials new nanomaterials (NMs) and molecularly imprinted polymer which matching six kinds of metal species would be synthesized according to their chemical characteristics, and employed to explore efficient selective recognition of indicator metal species, imprinting principle, adsorption law and dissolution methodologies. The model of reactive mechanism between NMs and related metal species was proposed to build up. A flow chart programme of speciation of off-line to on-line (O2O) for chemical identification and detection of metals species was planned to set up based on self-assembled NMs coupled FI-HPLC/GC-ICP-MS/AFS/GFAAS hyphenated technology. The metabolic effect and poison mechanisms of different metals species exposed to living environment was studied based on metabonomics method through animal test coupled NMs-uHPLC-ESI-MS-MS hyphenated instrument. Metabolic pathways of metals species were discussed and metabolic biomarkers were obtained to serve for new biometric monitoring method. These results would provide new theoretical and technical data for heavy metals toxic analysis, food safety and environmental/occupational health.