基于转录组测序研究的东南景天SaHMA3基因在镉解毒中的作用

关于镉超积累植物东南景天镉积累及解毒的作用机制虽然已有大量生理的研究，但是对其分子机制的研究还是空白。本项目在前期大量研究的基础上，拟通过转录组学测序获取东南景天转录组序列信息，构建东南景天镉解毒的代谢通路，系统性的分析东南景天镉解毒机理，并结合转录组测序结果及同源克隆手段来克隆镉解毒相关基因（SaHMA3），并综合利用RT-PCR、GFP融合表达、酵母功能互补、Immunohistology、基因过量表达等分子生物学手段及镉荧光探针、SEM-EDXMA、ICP-MS分析等植物生理学研究手段研究该基因的功能及其在东南景天镉解毒过程中的作用。研究结果可以为东南景天镉解毒的相关机制的研究提供理论依据，为挖掘植物镉超积累过程相关关键基因，创造出更适合于植物修复应用的新型超积累植物提供技术支持，这对于植物修复技术的推广发展，修复土壤重金属污染，保障生态环境和农产品安全都具重要意义。

Comparatively numerous researches had been done to study the physiology mechanisms of Cd tolerance and hyperaccumulation in Sedum alfredii-a chinese native Cd/Zn hyperaccumulator. However, the molecular mechanisms of Cd tolerance and hyperaccumulation are almost blank. Here, RNA-Seq technology will be used to obtain the sequence information of transcripts, to construct the metabolic passway of the Cd detoxification and to analysis the molecular mechanism of Cd tolerance in Sedum alfredii. Then a Cd tolerance related gene SaHMA3 will be cloned by homologous cloning combining the analysis of the transcriptome sequences.The function of this gene will be studied by multi-descipline technologies, such as RT-PCR, yeast heterologous expression, GFP fusion expression, immumohistology and overexpression as well as Cd fluorescent probe, SEM-EDXMA, ICP-MS analysis, etc.The aim of the research is to understand the molecular mechanism of Cd tolerance in Sedum alfredii, and to provide theoretical foundation for breeding new plants tolerant to Cd pollution, thus to lay the foundation for further in-depth study of the molecular mechanism of Cd hyperaccumulation in Sedum alfredii. The success of this research will be good for decreasing Cd pollution of envrionment and ensuring food safety, the sustainable development of agriculture and ecosystem health.