本项目选择真核浮游植物中具有代表性的藻华爆发原因种为研究对象，基于人类活动对全球气候及海洋环境影响加剧，磷源的相对输入量和生物可利用性都正在发生变化，针对“典型真核浮游植物对有机磷的利用、磷代谢途径和营养策略”这一科学问题，通过数据挖掘和生物信息学手段，拟初步构建真核浮游植物中磷代谢途径模式简图，并通过分析其与原核生物的异同点，探讨真核浮游植物在海洋中的磷营养生态策略。在此基础上，设计浮游植物培养实验，以磷酸酯和膦酸酯类可溶性有机磷作为唯一磷源，将观测到的生理响应和代谢途径结合分析，针对性地选择相应有机磷代谢途径中的转运蛋白和相关酶为研究目标，分析其在种间的表达调控机制差异。本研究从细胞代谢水平上系统了解具代表性真核浮游植物种类有机磷利用及磷代谢的分子机制，进而探讨其在自然海洋生境中磷营养策略和生态适应机制，以及浮游植物在磷的生地化循环中的角色。

The project will investigate the molecular mechanisms in marine eukaryotic phytoplankton to utilize dissolved organic phosphorus (DOP). The study will focus on representative bloom-forming species from different phyla of eukaryotic phytoplankton. Through data mining and comparative omics analysis, a draft phosphorus metabolic pathways in eukaryotic phytoplankton cells will be constructed, which will be used to perform further comparative research against equivalent pathways in prokaryotes. The main goal of the analysis is to identify the differences between eukaryotic and prokaryotic cells to gain understanding on the nutritional strategies in eukaryotpic phytoplankton. Moreover，a series of incubation experiments will be carried out using different types of DOP (phosphoesters and phosphonates) as the sole P source. Physiological status of the different cultures will be monitored daily and used as guide to look for potential functional key enzymes as well as transporters in the metabolic pathways. Further experiments will be conducted to look into the gene expression dynamics of those target genes under different P conditions, and the results will be integrated to elucidate different mechanisms among different species in utilizing these forms of DOP. This project will represent the most systematic research effort on the capacity and the molecular mechanisms of DOP utilization in eukaryotic phytoplankton, which will provide insights into P nutritional strategies and molecular mechanisms underlying ecological adaptation in the marine ecosystem as well as into the roles of these phytoplankton populations in biogeochemical cycles of phosphorus.