促肾上腺皮质激素释放激素(CRH)和尿皮质素 III（Ucn III）是同一家族的肽类物质，分别与CRH受体-1（CRH-R1）和CRH受体-2（CRF-R1）结合，在内分泌调节、自主神经活动和应激反应中起重要作用。应激反应不仅引起下丘脑室旁核（PVN）释放大量CRH，还影响PVN细胞活动和突触传递，甚至导致细胞功能紊乱和突触传递异常，但应激反应对PVN各种分泌细胞功能活动和突触传递的影响机制尚不完全清楚。因此，申请人将应用膜片钳、组织化学、单细胞RT-Multi-PCR和药理学手段，研究CRF和Ucn III对应激大鼠PVN催产素、加压素、CRH和神经介素U mRNA表达神经元电生理特征、局部环路及突触可塑性的影响，明确PVN分泌细胞上CRH-R1和CRH-R2在应激反应中的作用，阐明CRH和Ucn III对应激与非应激状态PVN分泌细胞活动、突触传递和突触可塑性的影响机制。

Corticotrophin-release hormone (CRH) and Urocortin III (Ucn III) are peptides of the same family that combines with CRH receptor-1 (CRH-R1) and CRH receptor-2 (CRH-R2), respectively. Both CRH and Ucn III are critical for regulation of neuroendocrine, autonomic nervous system activities and stress responses. Stress responses not only induce a release of CRH from parvocellular neuroendocrine cells in paraventricular nucleus (PVN) of hypothalamus, but also defeat the activity and synaptic transmission of PVN neuroendocrine cells, even result in a functional disorder and an abnormality of synaptic transmission of them. However, the mechanisms of the effects of strss responses on functional activities and synaptic transmission of PVN neuroendocrine cells are unclear. Thus, applicant will use patch-clamp recording, histochemistry，single-cell reverse transcription-multiplex polymerase chain reaction (sc-RT-Multi-PCR) and neuropharmacology methods to study the effects of CRH and Ucn III on electraphysiological properties, local neuronal circuits and synaptic plasticity of oxytocin, vesopression, CRH and neuromedine U mRNA expressing PVN neurons in stress rats. We aim to understand the functions of PVN neuroendocrine cell CRH-R1 and CRH-R1 during the stress responses, and to elucidate the mechanisms of the effects of CRH and Ucn III on the neuronal activity, synaptic transmition and plasticy in hypothalamic PVN in stress rats.