蛋白磷酸酶CRPPs调控植物感受和应答低温信号的分子机制

低温胁迫是限制植物生长的关键因子。目前，关于低温信号转导及相关调控因子的研究取得了一些进展。但是，植物如何感受低温信号并不清楚。磷酸酶-蛋白激酶级联系统通常在植物感受信号中发挥重要作用，我们前期筛选到两个编码蛋白磷酸酶基因的抗冻突变体crpp1/2。本研究中，我们将采用细胞生物学、遗传学和生物化学等方法系统研究CRPP在植物响应低温胁迫中的作用；深入探讨CRPP与低温信号中关键蛋白激酶OST1互作的生物学意义，揭示OST1低温激活的分子机制；进一步寻找与CRPP1互作的类受体蛋白激酶，为植物是如何感受低温提供直接的分子证据。本研究将有助于揭示植物感知和应答低温的分子奥秘，同时为分子育种提供重要的遗传资源。

Cold stress adversely affects plant growth and development. Although some progress has been made on cold transduction signaling pathway, how plants sense cold signal remains unclear. Based on the important roles of phosphatase-protein kinase cascade in perception and transduction of signals in plant, we screened the mutants of phosphatases, and identified crpp1 and crpp2. The loss-of-function mutants showed enhanced freezing tolerance. In this study, first we will characterize the biological function of CRPP1 and CRPP2 using genetic, biochemical, cellular and molecular approaches. Second, we will dissect the biological significance of the interaction of between CRPPs and OST1 protein kinase, and elucidate the mechanism of OST1 activation by cold stress. Finally, we will identify novel interacting-protein kinases of CRPP1, especially LRR-RLKs, which mediate plant freezing tolerance. This study will shed more light on the understanding of plant perception and response to cold stress, and provide genetic resource for the breeding of cold-tolerant crops.