BRASSINOSTEROID-INSENSITIVE2 Negatively Regulates the Stability of Transcription Factor ICE1 in Response to Cold Stress in Arabidopsis
BRASSINOSTEROID-INSENSITIVE2 Negatively Regulates the Stability of Transcription Factor ICE1 in Response to Cold Stress in Arabidopsis
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BRASSINOSTEROID-INSENSITIVE2 负调控转录因子 ICE1 响应拟南芥冷胁迫的稳定性
DOI：
10.1105/tpc.19.00058
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发表时间：
2019-08
期刊：
影响因子：
11.6
通讯作者：
Yang Shuhua
中科院分区：
文献类型：
--
作者：
Ye Keyi;Li Hui;Ding Yanglin;Shi Yiting;Song Chunpeng;Gong Zhizhong;Yang Shuhua
在冷胁迫下，蛋白激酶BIN2与CBF表达诱导剂1相互作用并磷酸化CBF表达诱导剂1，加速其蛋白酶体介导的降解，从而负调控抗冻性。寒冷适应是植物在冰冻温度下生存的关键策略。C-REPEAT BINDING FACTOR （CBF）基因是低温快速诱导的基因，在低温驯化过程中起着重要作用。然而，在冷胁迫反应的后期，CBF表达衰减的机制尚不清楚。在这里，我们发现在长时间的冷胁迫下，拟南芥（Arabidopsis thaliana）中的蛋白激酶brassinosteriinsensitive2 （BIN2）与CBF表达诱导剂（ICE1）相互作用并磷酸化，促进ICE1与E3泛素连接酶之间的相互作用，从而促进ICE1的降解。BIN2的激酶活性在冷应激反应的早期阶段受到抑制，随后恢复，这表明当CBF表达减弱时，BIN2主要下调ICE1的丰度。ICE1的功能缺失突变部分抑制了CBFs的冷诱导表达，并损害了bin2-3 bil1 bil2增强的抗冻能力。这些发现揭示了BIN2在调节CBF表达，从而平衡植物生长和冷胁迫反应中的重要作用。
The protein kinase BIN2 interacts with and phosphorylates INDUCER OF CBF EXPRESSION1 under cold stress, accelerating its proteasome-mediated degradation to negatively regulate freezing tolerance. Cold acclimation is a crucial strategy for plant survival at freezing temperatures. C-REPEAT BINDING FACTOR (CBF) genes are rapidly and transiently induced by low temperature and play important roles in cold acclimation. However, the mechanism underlying the attenuation of CBF expression during the later stages of the cold stress response is obscure. Here, we show that the protein kinase BRASSINOSTEROID-INSENSITIVE2 (BIN2) interacts with and phosphorylates INDUCER OF CBF EXPRESSION1 (ICE1) in Arabidopsis (Arabidopsis thaliana) under prolonged cold stress, facilitating the interaction between ICE1 and the E3 ubiquitin ligase HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE1 and thereby promoting ICE1 degradation. The kinase activity of BIN2 is inhibited during the early stages of the cold stress response and is subsequently restored, suggesting that BIN2 mainly downregulates ICE1 abundance when CBF expression is attenuated. A loss-of-function mutation of ICE1 partially suppresses the cold-induced expression of CBFs and compromises the enhanced freezing tolerance of bin2-3 bil1 bil2. These findings reveal an important role for BIN2 in fine-tuning CBF expression, and thus in balancing plant growth and the cold stress response.
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影响因子：
11.8
作者：
Zhao C;Wang P;Si T;Hsu CC;Wang L;Zayed O;Yu Z;Zhu Y;Dong J;Tao WA;Zhu JK
通讯作者：
Zhu JK
影响因子：
13
作者：
Eivers, Edward;Demagny, Hadrien;De Robertis, E. M.
通讯作者：
De Robertis, E. M.
影响因子：
7.4
作者：
Gilmour, SJ;Sebolt, AM;Thomashow, MF
通讯作者：
Thomashow, MF
MPK3 和 MPK6 介导的 ICE1 磷酸化负向调节拟南芥中 ICE1 的稳定性和冷冻耐受性
影响因子：
11.8
作者：
Li, Hui;Ding, Yanglin;Yang, Shuhua
通讯作者：
Yang, Shuhua
影响因子：
64.5
作者：
Yin, YH;Vafeados, D;Chory, J
通讯作者：
Chory, J