植物细胞质雄性不育现象普遍存在。研究证实，线粒体编码基因的表达参与细胞质雄性不育形成的调控。棉花杂种优势显著，能够利用细胞质不育系培育三系杂交种，但其雄性不育的分子机制仍不清楚。本研究利用棉花细胞质雄性不育三系材料，基于三系线粒体基因组分析，确定候选基因/orf；结合线粒体转录组差异表达分析，进一步缩小候选基因范围，对候选基因构建线粒体靶向载体进行遗传转化及转基因验证。同时，利用转录组测序、lncRNA和miRNA测序数据，结合线粒体蛋白质组分析，筛选棉花细胞质雄性不育形成相关候选基因和蛋白，确定不育基因转基因验证其功能；根据转基因后代表现，分析不育相关基因表达及调控机制，构建棉花细胞质雄性不育分子代谢调控网络，阐明棉花细胞质雄性不育形成的分子机制。

The cytoplasmic male sterility (CMS) is widespread in plant, and some genes and orfs of plant mitochondrial genome are involved in the sterility and sterile recovering in many plants. Cotton is one of the crops that have heterosis and can be used CMS system to develop hybrids but the molecular mechanism of sterility remains unclear. To uncover the molecular mechanism of cytoplasmic male sterility in cotton, our research will base on mitochondrial genome sequences newly obtained. Comparing the completely mitochondrial genome sequences of CMS lines, maintainer and restorer lines of upland cotton, we aim to identify cytoplasmic male sterility-related candidate gene and proteins in cotton, combining with analyzing the mitochondrial transcriptome, mitochondrial proteome. The CMS associated genes/orfs are further targeted, and the candidate CMS-associated genes/orfs with complete reading frame are constructed into mitochondria targeted vectors for transformation to validate the functions. Using the information of transcriptome as well as mitochondrial genomes, we analyze the function of regulation molecules, such as lncRNA and miRNA, and how these molecules regulate the CMS-associated genes/orfs, then to select the target candidate genes and using to construct vectors, and to transform cotton plants; Based on the evaluation results on the positive transformed plants and the offspring, we try to explore the function of sterile genes, and to outline the metabolism regulatory network in cotton CMS line, and to clarify the molecular mechanism of cotton cytoplasm male sterility.