蜱类是专性吸血的外寄生动物，传播多种疾病，给人类健康和畜牧业带来极大危害。目前控制蜱类的主要手段是使用化学杀虫剂，长期使用引起蜱的抗药性，同时造成环境污染，因此，抗蜱疫苗的研制已成为研究热点。副肌球蛋白(Pmy)是存在于无脊椎动物中的一种肌纤维蛋白，由于脊椎动物体内无此同源性蛋白，不会引起自身免疫反应，因此，它是最理想的疫苗候选抗原之一。本项目拟以长角血蜱Haemaphysalis longicornis为研究对象，在课题组前期成功克隆其Pmy基因的基础上，运用生物化学、分子生物学、免疫学等现代生物学的研究方法，对长角血蜱Pmy基因表达、蛋白纯化及免疫原性等方面进行系统研究，并将不同型式Pmy疫苗免疫接种动物，通过抗蜱实验鉴定其功能。研究结果为制备抗蜱疫苗提供依据，对于揭示蜱类免疫应答机制以及蜱类防治具有重要的理论意义和实际应用价值。

Ticks are obligate hematophagous ectoparasites, and transmit many diseases, causing heavy damage to human health and livestock. Tick control is principally dependent on the application of acaricides. However, it can develop resistance to acaricides for ticks and raises environmental contamination. So, more and more researches have been focused on the vaccine against ticks. Paramyosins are myofibrillar proteins，and exist in invertebrates. Meanwhile, the vertebrates don't have homologous paramyosins in the body, and it can't cause autoimmune responses. Thus, paramyosins are one of the most ideal candidate antigen for vaccine. In early stage, we successful cloned the gene of paramyosins in Haemaphysalis longicornis. In this study, we have an system research on the gene expression, protein purification and immunogenicity of paramyosin in H. longicornis, by using the method of biochemistry, molecular biology, immunology and so on. In order to identify the immune function, we will do the experiment of animal vaccination by using different kinds of Pmy vaccine. The results could be the evidence for vaccine against ticks. This study not only has important theoretical significance for revealing the mechanism of immune response in ticks, but also has practical application value for tick control.