鼻咽部共生细菌作用下鼻咽上皮细胞的改变及其表观遗传学机制

我们的前期研究利用非培养的方法发现鼻咽部有独特的微生物环境和一些特有的菌种。我们提出假设，在鼻咽部特有的共生细菌作用下，鼻咽上皮细胞发生表观遗传学改变，这些改变可能在鼻咽上皮细胞的多步骤癌变中扮演一定角色。因此，本研究将利用前期工作中筛选出的鼻咽部特有的共生细菌处理体外培养永生化的鼻咽上皮细胞，建立鼻咽上皮细胞与鼻咽部共生细菌共培养的模型，观察上述细菌对鼻咽上皮细胞生物学行为的影响，并探索鼻咽上皮细胞在这些细菌营造的微环境下发生怎样的表观遗传学改变。为进一步研究在环境致癌物、鼻咽部的特殊微生境和EBV的交互作用下，正常鼻咽上皮细胞多步骤癌变机制提供线索和基础数据。

In our previous studies, through a culture-independent method, we identified that there is a unique micro flora in the human nasopharynx, which contains some speical bacteria species. We hypothesis that treated by these unique species, nasohpayngeal epithelia cells might be regulated by epigenetic mechanisms. The regulation might play a role in the multi-step process of nasopharyngeal carcinogenesis. In this study, we will treat the immortilized nasopharyngeal epithelia cells with those special bacteria species which are selected by our previous study and setup a co-culture modle. We will observe the impact on the biological behavior changes and to explore epigenetic regulation of nasopharyngeal epithelia cells under the microenvironment created by such bacteria species. The study will provide valuable clues and basic knowledge for further research of the mechanism of multi-step nasopharyngeal carcinogenesis driven by the interaction of environmental carcinogens, the unique nasopharyngeal microflora, and EBV.