现有研究表明，不仅癌细胞在发生发展过程中积累了较高的体细胞突变，事实上，我们身体内的所有细胞都有不同程度的基因独特性，这一点目前已获得科学研究者的广泛共识。然而，现有的核酸分析仍然以细胞群体为主要研究对象，忽略了细胞间的个体差异，难以满足日新月异的生物学与医学研究需求。针对上述问题，结合本实验室研究特色，本项目提出高通量数字化单细胞阵列核酸分析平台研究：采用选择性微流控液滴发生技术实现单细胞包裹，结合基于栅栏结构的水力捕获技术，构建数字化单细胞分析阵列，以环介导等温扩增为分析手段，实现外周血单核细胞高通量、快速、准确的核酸分析与诊断，为CTC循环肿瘤细胞突变筛查提供一个创新途径，建立一个具有普适意思的高通量单细胞核酸分析平台。本课题对于高通量单细胞分析具有重要科学价值，并促进生物物理学、细胞生物学、医学及其相关交叉学科的发展和人才培养。

Existing studies have shown that not only cancer cells have accumulated high somatic mutations in the course of development, but actually, all cells in our body have different degrees of gene specificity. This has been widely recognized by scientific researchers. However, current methods for nucleic acid analysis still takes cell population as the main research object, neglecting the individual differences among cells, which is difficult to meet the needs of the emerging biological and biomedical research. In view of the above-mentioned problem and combining with the research features of our laboratory, this project proposes a microfluidic digital single-cell assay for high-throughput nucleic acid analysis. By employing selective droplet generation technique in coupling with fence-based hydrodynamic capturing, microfluidic digital single-cell assay can be established. With loop mediated isothermal amplification for nucleic acid analysis, it will open up a new avenue for mutation screening of circulating tumor cells from a population of mononuclear cells with reduced analysis time and enhanced throughput. The established method will provide a universal platform for high-throughput single-cell nucleic acid analysis. This study is of great scientific value for high throughput single cell analysis, and promotes the development of biophysics, cell biology, medicine and related interdisciplinary development and personnel training.