高灵敏液相生物芯片检测系统及其在肺癌早期诊断中的应用

我国首位恶性肿瘤死因是肺癌。这一严重现状与临床上缺少灵敏、特异的早期实验室诊断标志物和方法密切相关。因此，本项目拟在前期研究基础上将表面包覆贵金属纳米壳层的量子点荧光微球结合流式细胞仪构建出蛋白和microRNA高灵敏液相生物芯片多元检测双平台；采用SPG膜乳化法结合种子生长法和pickering乳液法探索包覆贵金属纳米壳层量子点荧光微球的可控制备;研究其在流式细胞仪上实现编码和解码的方法和途径，同时研究利用贵金属荧光共振增强效应获得高检测灵敏度；选择目前临床常用的肺癌肿瘤标志物和新发现的肺组织相关的micro RNAs作为标志物，探索并建立稳定性好、灵敏度高和线性范围宽的多指标检测技术平台；经临床样本检测和ROC曲线分析评价检测性能和临床价值；为建立肺癌早期诊断专家分析系统奠定基础，为肺癌早期诊断提供高灵敏度、高特异性，便捷和快速的检测方法与技术平台，使更多的肺癌患者能从该技术中受益。

Lung cancer is the main reason of death among all the cancer patients, which is closely related to limited effective biomarkers and diagnosis methods. This project attempts to combine quantum dots encoded fluorescent microspheres coated by noble metal nanoshells with controllable optical properties and the ordinary flow cytometry to build a novel high sensitive liquid biochip double-platform for proteins and microRNAs. SPG membrane emulsification combined with seeded growth method and pickering emulsion method will be used to achieve the controllable preparation of quantum dots encoded fluorescent microspheres coated by noble metal nanoshells. After successfully achieving the ways of encoding and decoding, and investigating the effect of metal enhance fluorescence on detection sensitivity, the novel suspension liquid biochip platform will be used to realize high-throughput, fast joint detection of lung cancer specific protein and miRNA biomarkers, respectively, which will lay the foundation for further development of novel suspension liquid biochip with independent artificial intelligence, and provide an efficient new way for early diagnosis of lung cancer.