钙黄绿素（Calcein, CAL）作为一种简单、有效的化学标记物，已被广泛应用于标记鱼类、贝类等水生动物，已有的研究表明CAL能在多种鱼类的钙质结构上形成荧光标记。然而，由于缺乏有关CAL在鱼体内代谢和残留的科学研究证据，使得该简便有效的标志技术难以推广使用。本项目拟以鲢（Hypophthalmichthys molitrix）幼鱼为供试鱼类，研究浸泡、注射、投喂三种处理方式下，不同剂量/浓度CAL对鲢幼鱼的毒性效应和作用机理为基础，考察CAL在鲢幼鱼肌肉、肝脏、肾脏等组织器官与骨骼、鳞片、鳍条等钙质骨质结构中的蓄积和残留情况，运用药物代谢动力学模型，综合不同处理方式、CAL剂量/浓度、作用周期等因素，揭示CAL在鱼体内的代谢动力学过程和残留特征。本研究可为全面探明荧光染料在鱼体内的代谢、残留和消除机制奠定基础，对推动我国渔业资源科学管理、生物多样性保护具有重要的理论和实践意义。

Calcein (CAL), which is known as an important fluorochrome labeling dye, has been widely applied in marking fish and shellfish. Previous studies have proven that CAL was effective in producing fluorescent or pigmented marks in calcified and bony structures of many freshwater and marine fish species. Due to the lack of research concerning metabolism and residues of CAL, CAL mark has not been promoted in public waters, especially for food fish. Therefore, our present study plans to 1) study the toxic mechanism of CAL for juvenile silver carp, Hypophthalmichthys molitrix, by two application methods (i.e., immersion, injection, and feeding), 2) quantify CAL residues in different tissues (including blood, muscle, liver, kidney, and brain, etc.) and bony structures (including sagittae, scale, fin ray, and bone, etc.) of silver carp, and 3) use pharmacokinetic model to explain the distribution, excretion and accumulation mechanisms of CAL in silver carp. These results will provide a basis for revealing the metabolism and residues of fluorochrome labeling dyes in fish body, and it will be helpful for further supervision of fluorescent labeling application in stocking.