Effects of calcein on the levels of antioxidant enzymes and lipid peroxidation in juvenile silver carp, Hypophthalmichthys molitrix
Effects of calcein on the levels of antioxidant enzymes and lipid peroxidation in juvenile silver carp, Hypophthalmichthys molitrix
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钙黄绿素对鲢鱼幼鱼抗氧化酶和脂质过氧化水平的影响
DOI：
10.1111/are.15032
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发表时间：
2021-04
影响因子：
2
通讯作者：
Yao Weizhi
中科院分区：
文献类型：
--
作者：
Lu Hongjian;Chen Linghan;Xie Zhenhui;Fu Mei;Su Shengqi;Yao Weizhi
采用标准单次暴露方法，研究了钙黄绿素（CAL）对幼银抗氧化酶水平和脂质过氧化的影响。幼鱼暴露于不同浓度的CAL溶液（0，100，300，500和700 mg/L）的不同浸泡时间（12，24，48，72和96小时）。谷氨酸-草酰乙酸转氨酶、谷氨酸-丙酮酸转氨酶和碱性磷酸酶活性增加被认为是由于CAL诱导的肝损伤，可能是因为CAL在鱼肝胰腺中蓄积的可能性很高。CAL浓度较高（>300 mg/L）和浸泡时间较长（>48 h），对幼银鲤的毒性较大，并对鳃和肝胰腺造成一定程度的损伤。对于CAL暴露≤24小时，鳃和肝胰腺中的抗氧化反应可成功消除过量CAL诱导的活性氧。结果表明，CAL对幼银鲤抗氧化酶水平和脂质过氧化的毒性效应与CAL浓度和暴露时间有关。用CAL标记幼银鲤时，CAL溶液浓度应≤300 mg/L，浸泡时间应≤24 h。
Standard single‐exposure of calcein (CAL) was conducted on juvenile silver carp,Hypophthalmichthys molitrix, to examine its effects on antioxidant enzyme levels and lipid peroxidation. Juveniles were exposed to different concentrations of CAL solutions (0, 100, 300, 500 and 700 mg/L) for different immersion periods (12, 24, 48, 72 and 96 hr). Increased glutamate‐oxaloacetate transaminase, glutamate‐pyruvate transaminase and alkaline phosphatases activities were assumed to be due to CAL‐induced hepatic damage, probably because of the high potential of CAL to accumulate in fish hepatopancreas. Higher CAL concentrations (>300 mg/L) and prolonged immersion periods (>48 hr) could lead to greater toxicity in juvenile silver carp and, to a certain degree, damage the gills and hepatopancreas. The antioxidant responses in the gills and hepatopancreas result in successful elimination of the excess CAL‐induced reactive oxygen species for CAL exposure ≤24 hr. Our results confirmed that the toxic effects of CAL on antioxidant enzyme levels and lipid peroxidation in juvenile silver carp depended on both CAL concentrations and duration of exposure. Moreover, concentration of CAL solutions and the immersion period should be, respectively, restricted to ≤300 mg/L and ≤24 hr, when CAL is used for immersion marking juvenile silver carp.
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