真菌毒素是由某些丝状真菌产生的有毒代谢产物，严重危及农产品安全。三唑类手性杀菌剂广泛用于真菌病害防治，其对映体具有选择性活性，但是否选择性调控真菌产毒素尚不清楚。项目前期研究表明，戊唑醇、腈菌唑对映体会选择性影响镰刀菌产真菌毒素。基于此，本项目选取重要产毒性病原禾谷镰刀菌和串珠镰刀菌为目标菌株，在测定三唑类杀菌剂对映体生物活性基础上，结合环境因素开展对映体选择调控镰刀菌产毒素研究，采用组学等技术，研究菌株氧化应激、毒素合成相关基因和蛋白的表达与互作、特定蛋白与对映体结合等，结合代谢产物分析，阐明选择性调控机制。本项目创新之处在于首次系统研究三唑类手性杀菌剂对映体选择性调控真菌产毒素及其机制。项目结果将有助于深入解析真菌毒素合成机制，为真菌毒素污染控制提供新思路，为筛选安全高效杀菌剂提供理论指导，完善三唑类手性杀菌剂和真菌毒素风险评估数据，对于确保农产品安全具有重要的理论和现实意义。

Mycotoxins, which seriously endanger to the safety of agricultural products, are kinds of toxic secondary metabolites produced by some filamentous fungi. Chiral triazole fungicides were widely used to control the crop fungal diseases. The enantiomers often show different bioactivity to the target fungi. However, it is no reports about enantiomers can selectively regulate the production of mycotoxins by toxigenic fungi. Our study showed that tebuconazole and myclobutanil enantiomers had different bioactivity to Fusarium graminearum and F. moniliforme and could selectively effect on mycotoxins production. In this study, two important toxigenic pathogens Fusarium graminearum and F. moniliforme are selected to study as target strains. Firstly, stereoselective bioactivity of enantiomers to two toxigenic Fusarium spp. and selective affect on production of mycotoxins are determined. The in vivo changes of the strains, the expression and interaction of genes and proteins related to mycotoxin synthesis, and the docking of enantiomers and proteins were studied to clarify the mechanism of selective regulation by means of metabolomic, genomics and proteomics methods. The most important significance of the study is the first systematic studies on chiral triazole fungicides enantiomers differentially regulate the mycotoxins synthesis by toxigenic fungi. The results will provide more informations about the mechanism of mycotoxin production and provide new ideas for control the mycotoxins contamination in agricultural products and theoretical basis for screening safe and efficient fungicides, and provide more datas for the risk assessment of chiral triazole fungicides and mycotoxins in agro-products. It is very important theoretical and practical significance to ensure the safety of agricultural products.