ARL15通过AKT/Adipoq通路调控猪饲料转化率性状的分子机制研究

饲料转化率性状是影响生猪养殖效益的关键因素之一，目前关于饲料转化率性状分子机制解析鲜有报道。脂联素Adipoq是影响猪饲料转化率性状的关键因子之一。我们前期研究发现ARL15基因与饲料转化率性状显著相关，且ARL15可以调控Adipoq的表达和分泌。综合分析推测ARL15是通过AKT信号通路调控Adipoq的表达进一步影响饲料转化率性状。在此基础上，本项目首先解析ARL15转录调控模式及胰岛素对ARL15、AKT和Adipoq调控关系；其次，验证脂肪细胞内ARL15对AKT信号通路直接调控作用，鉴定通路中关键蛋白和磷酸化激酶。最后，综合分析并验证ARL15通过AKT信号通路调控Adipoq表达的作用机制及其与饲料转化率的关系。系统阐述ARL15通过AKT/Adipoq通路调控猪饲料转化率性状内在的分子机理。上述研究将对提高猪饲料转化效率，节能减排，提高生猪产业效益提供重要的依据。

Feed conversion ratio trait is one of the crucial factors involving in pig industry benefit. Until now, less study was reported about the understanding of pig feed conversion ratio trait. Adiponectin is one of the important factors responsible for feed conversion ratio trait. In our previous study, significant association event was identified between ARL15 gene and feed conversion ratio trait, and ARL15 was also found to regulate the expression and secretion of Adipoq (Adiponectin) in adipocytes. Based on combined analysis, we predicted that ARL15 regulated the Adipoq expression through AKT signal pathway and further influenced the pig feed conversion ratio trait. On this basis, we firstly uncover the transcriptional and regulatory pattern of ARL15 and regulatory effect of insulin on ARL15, AKT and Adipoq. Subsequently, direct regulatory effect of ARL15 on AKT signal pathway was validated and key proteins and phosphorylated kinases were identified. Lastly, regulatory mechanism of ARL15 on Adipoq via AKT signal pathway was studied and association between adipoq and feed conversion ratio was also identified. This study will uncover the regulatory mechanism of ARL15 on pig feed conversion ratio via AKT/Adipoq signal pathway. In general, above results will provide the important evidence for improving pig feed conversion efficiency, conserving energy, reducing emissions, and improving pig industry benefit.