RNA动态修饰的化学调控

本研究基于申请人发展的光促进的RNA修饰的化学调控，通过设计新颖的化学生物学的研究技术和干预手段，针对RNA在不同状态（时间、空间）下的动态修饰开展高效、高选择性和高灵敏度的化学修饰与调控的研究。一方面根据已知的修饰酶和去修饰酶，设计、合成结构可控的仿生化学催化体系，发展条件温和、效率较好、生物容忍度高的化学转化反应，在活细胞中实现RNA修饰的精准操纵。另外，拟利用化学小分子可控地制造RNA修饰突变，实现分子水平的RNA进化模拟，为研究发展具有实际应用价值的RNA修饰干预机制提供重要科学基础，为发现RNA修饰新的生物学功能提供关键线索。

In this project, we will focus on the development of novel chemical biology techniques for RNA epigenetics research. This method has several advantages comparing with traditional procedures, in which dynamic RNA modification could be modulated at different conditions with high efficiency, selectivity and sensitivity. The design and evaluation of chemical modulation approaches will be based on the known structures of modification enzymes, and the so developed biomimetic catalytic system would benefit the modulation of RNA modification in living cells. Besides, the use of chemical molecules will accelerate the evolution of RNA mutation and hence change the fate of the cell.