在肿瘤转移过程中，细胞运动侵袭是肿瘤细胞最基本的生物学行为。细胞的迁移运动分为单细胞运动和群体运动。大部分侵袭性实体瘤主要表现为群体性侵袭运动，但是肿瘤群体细胞运动的机制仍不清楚。本项目组前期研究表明CD147诱导基质金属蛋白酶分泌降解细胞外基质，促进肿瘤细胞的浸润转移。在单细胞运动过程中，CD147能激活integrin下游信号通路，促进粘着斑生成，伪足增加，并通过Rho/Rac信号通路调控肿瘤细胞运动形式的转换。CD147是否参与群体细胞运动目前未见报道。前期研究显示在matrigel 3D培养条件下，CD147高表达并增强肝癌细胞间黏附、克隆性生长、与成纤维细胞的相互作用、侵袭和转移的潜能。本课题拟利用体外3D细胞培养体系、动物体内模型以及临床病理样本多体系多方法研究肿瘤群体细胞运动的特征，阐明CD147分子在肿瘤群体细胞运动中重要分子机制，为新的肿瘤靶向治疗策略提供重要线索和依据。

In the metastatic process of cancer cells,cell motility and invasion are the main biological behaviors.There are two modes of cell motility,individual cell motility and collective cell motility.Collective cell motility is the preferential mode for most invasive solid tumors.But the precise regulation mechanism is not clear.Our previous studies showed that CD147 stimutates MMPs secretion to degrade extracellular matrix and promotes cancer invasion and metastasis.In the process of individual cell motility,CD147 promotes focal adhesion and invasive pseudopodia formation by activating integrin signal pathways.CD147 also regulates switching between modes of motility via Rho/Rac signal pathways.But it has been not reported whether CD147 is involved in the regulation of collective motility of cancer cells.our reviours results showed that expression of CD147 was enhanced under the 3D cell culture condition and promoted cancer cell-cell contact,clonal proliferation, interaction with fibroblasts and potentials of invasion and metasitasis.In this study, we use the 3D cell culture system,in vivo animal model and clinical tissue samples to explore the features of collective cancer cell motility and clarify the role of CD147 in the regulation of collective cancer cell motility,and give rise to the promise of tageting therapeutic effects of this molecule in HCC treatments.