我国特别是浙江金属污染日益严重，浙江省近年兴起了“鱼塘转荷塘”治理方式，实现经济发展和环境治理双赢。但原鱼塘的金属污染会在底泥与莲藕之间发生迁移、形态转化和蓄积，带来健康风险。金属的毒性不仅与总量息息相关，更与具体的存在形式及浓度有关。本项目将开发一种在线固相萃取富集的HPLC-ICP-MS方法，实现ng/L水平的铬、砷、镉、汞和铅的同时形态分析，大大提高HPLC-ICP-MS的分析速度和样品通量，显著降低多种金属形态分析的成本和环境废物。在此基础上，对杭嘉湖地区荷塘生产的莲子、藕带和莲藕等农产品中铬、砷、镉、汞和铅的形态进行分析，评价其对人体的健康风险。更进一步，对不同时间采集的荷塘的塘水、底泥、荷叶、莲子和莲藕样品进行铬、砷、镉、汞和铅的形态分析，研究它们的不同形态在塘水、底泥 和莲藕之间的迁移、转化和蓄积等变化规律，为制定和实施防治措施以及荷塘出产食品的食品安全监控奠定基础。

Metal pollution is growing more and more seriously especially by taking a typical case of severe water pollution in Zhejiang Province. To counteract deleterious influence from heavy metals to ponds, a preventive and treated strategy namely transformation of fish ponds to lotus ponds is continuously adopted for both sakes of economic development and environmental governance. However, original metal species in polluted fish ponds may undergo transport, transformation and accumulation from pond water and sediment to lotus (especially lotus rhizome and lotus seed), which may lead to a high risk of human health. Toxicity of one certain metal is not merely dependent on its total content, but is mainly decided by chemical forms involved and their concentrations. This proposal aims at simultaneous speciation analyses of chromium, arsenic, cadmium, mercury and lead at ng/L levels by high performance liquid chromatography hyphenated with inductively coupled plasma mass spectrometry (HPLC-ICP-MS) after online preconcentration by solid phase extraction. Analytical speed and sample throughput of HPLC-ICP-MS methodologies are hence enhanced significantly while operation cost and reagent waste are both dramatically decreased. On this basis, speciation analyses of chromium, arsenic, cadmium, mercury and lead to lotus products such as lotus root silk, lotus rhizome and lotus seeds are thereafter carried out to estimate their healthy risk. The HPLC-ICP-MS method is further applied to determine various metal species of chromium, arsenic, cadmium, mercury and lead in pond water, sediment, lotus leaves, lotus rhizome and lotus seeds which are sampled from lotus ponds in different days. Transport and transformation patterns of chromium, arsenic, cadmium, mercury and lead species among pond water and sediment, and lotus will be elucidated accordingly, which may guide designing and execution of corresponding actions to treat polluted ponds as well as safety estimation of lotus products.