低发土传枯萎病蕉园根际微生物区系特征解析及其调控机制

低发土传枯萎病蕉园根际微生物区系特征解析是防控香蕉连作生物障碍理论基础。申请人前期在海南省广泛调查获得长期连作低发枯萎病蕉园，但根际微生物区系特征及其如何由土体微生物区系孵育形成亟待研究。前期研究同时证实石灰+碳铵熏蒸联用生物有机肥技术能够有效防控连作香蕉枯萎病的发生，进而形成低病蕉园，但熏蒸联合生物有机肥施用造成的土壤微生物区系变化、调控后的根际微生物区系与调查所得低病蕉园抑病根际微生物区系之间的关联性还有待深入研究。本项目拟利用MiSeq测序技术分析土壤微生物具体类群，辅以Real-Time PCR技术测定微生物数量，分析阐明大尺度调查所得低发病蕉园根际微生物区系特征及其由土体微生物区系孵育形成机制，结合石灰+碳铵熏蒸联用生物有机肥调控所成土体及根际微生物区系特征，探明调控土体土壤微生物区系形成抑病根际微生物区系的机制，最终为防控香蕉连作生物障碍提供理论依据。

Analysis of rhizosphere microflora in banana plantation with low fusarium wilt disease incidence could provide the theoretical basis for preventing banana continuous cropping obstacle. Banana orchards that still maintain a low banana Fusarium wilt disease incidence after consecutive years of banana planting have been found in the Hainan Island, China. However, the characteristics of the rhizosphere microflora and its formation mechanism from bulk soil in these low-disease banana plantations are still unknown. Previous studies have demonstrated that combined application of fumigation using ammonium bicarbonate mixed with lime and bio-organic fertilizer could effectively suppress the disease. However, what kind of microbial community created after fumigation and bio-organic fertilizer application and what mechanism involved in rhizosphere microflora reconstruction from bulk soil need deep investigation. In addition, the relationship between rhizosphere microflora from investigation and regulation also need deep investigation. In this project, MiSeq sequencing technique will be used to analyze the specific microbial community and structure. Real-Time PCR technique will be used to detect the number of microbes. Then the characteristics of the rhizosphere microflora and its formation mechanism from bulk soil in low-disease banana plantations from investigation and regulation will be studied. This project will find out the mechanism involved in rhizosphere microflora reconstruction from bulk soil and provide an important theoretical basis for preventing the continuous cropping obstacle of banana planting.