设施番茄抑病型土壤微生物区系形成与维持机制的解析是保障其可持续生产理论基础。申请人前期已通过连续10季番茄田间试验，确认了生物有机肥的防病效果，并证实了生物有机肥通过调控形成抑病型土壤微生物区系防控青枯病的发生。但生物有机肥驱动的番茄抑病型土壤微生物区系形成机制依然未得到系统研究，所构建的抑病型土壤微生物继续随生育期和种植季节的变化规律及其耦联的功能维持机制还未得到研究。本项目以长期田间试验定位点为依托，基于已人为调控形成的抑病型土壤，解析抑病型土壤微生物区系的形成机制，探明关键微生物的形成特征；揭示所形成抑病型土壤微生物区随继续种植的变化规律与功能维持机制，阐明关键微生物菌群在变化过程中的抑病作用；同时，将利用宏培养组技术结合高通量数据的多元统计分析和室内盆栽测试，建立功能明确的关键微生物资源库，构建高效菌群组合，并解密其联合有机添加物的土壤微生区系调控机理，最终为实际调控提供理论借鉴。

The analysis of the formation and maintenance mechanism of tomato disease suppressive soil microbiota is the theoretical basis for ensuring the sustainable production. The applicant has demonstrated the bio-control efficiency of bio-organic fertilizer in a long tomato field experiment through 10 years continuous application by the formation of disease-suppressive soil microbiota. However, the formation mechanism of disease suppressive soil microbiota resulted from bio-organic fertilizer application has not yet been explored, and the subsequently variation of the disease suppressive soil microbiota associated with the maintenance mechanism of disease suppression function along with the growth period and planting season has not been studied. Based on the long-term field experiment and the formed disease suppressive soil, this project will analyze the formation mechanism of disease-suppressive soil microbiota, and identify the formation characteristics of the key microorganisms; and will reveal the variation of microbiota in the formed disease suppressive soil and decipher the associated mechanism of disease suppression maintenance along with the growth period and planting season. Meanwhile, this project will construct key microbial resource banks with clear function by Meta-cultural method, combined with the multi-statistical analysis of high-throughput data and the indoor pot experiment. After that, the project will construct microbial combinations with high-efficient disease suppression ability, after that regulation mechanism of the microbial combinations combining organic additives for formatting disease suppression soil microbiota will be subsequently explored, ultimately providing the theoretical basis for the actual regulation of soil microbiota.