多种植物激素的同时检测方法学研究

本项目将围绕多种超微量植物激素的同时检测方法学开展研究。针对植物中植物激素种类多、化学性质差异大、含量低等特点，拟通过两种途径开展研究工作。途径一为通过研发多种新型分离介质，发展串联模式样品前处理技术，对植物激素进行分类富集纯化，利用申请人已经建立超微量植物激素检测方法，获取多种植物激素的信息；途径二拟发展多维毛细管整体柱微萃取技术、超长杂化硅胶整体柱分离技术、柱后衍生质谱检测技术等实现多种植物激素的同时检测。项目的实施可望建立拟南芥和水稻等植物中多种植物激素的同时检测平台，为我国植物学家的相关研究，提供稳定可靠的多种植物激素测定数据，也将促进分析化学与植物科学交叉学科研究的发展。

The project will focus on the development of methodology for simultaneous and sensitive determination of various types of trace endogenous phytohormones. In view of the distinct chemical properties and low endogenous contents of a variety of phytohormones, we propose two strategies to carry out the research work. As for Strategy 1, we plan to develop diverse novel separation media and establish tandem mode of sample pretreatment techniques to enrich and purify phytohormones with different categories. By coupling the sample pretreatment strategies developed in this project and the ultra sensitive detection methods previously established in our group, we will obtain abundant information for many kinds of endogenous phytohormones. As for Strategy 2, we plan to develop microextraction methods using multi-dimensional capillary monoliths and establish separation technology using ultra-long hybrid silica monolithic columns with post-column derivatization, with which simultaneous and sensitive determination of multi endogenous phytohormones will be achieved by coupling with mass spectrometry analysis. The implementation of the project is expected to establish a platform for the simultaneous determination of a variety of phytohormones in plants such as Arabidopsis and rice, which will provide reliable measurement of multi endogenous phytohormones for the related research in China and promote the interdisciplinary development between analytical chemistry and plant science research.